| 六种抗大鼠Nogo-A分子单克隆抗体的免疫荧光组织化学染色特性的比较 |
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| 引用本文： | 邓斌,刘芳芳,赵湘辉,于才勇,卞干兰,冯睿,鞠躬,王键.六种抗大鼠Nogo-A分子单克隆抗体的免疫荧光组织化学染色特性的比较[J].细胞与分子免疫学杂志,2011,27(4):425-427. |
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| 作者姓名： | 邓斌 刘芳芳 赵湘辉 于才勇 卞干兰 冯睿 鞠躬 王键 |
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| 作者单位： | 第四军医大学神经生物学教研室,陕西,西安,710032 |
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| 基金项目： | 国家重大新药创制大平台项目子课题(2009ZX09301-009-PT02); 国家自然科学基金资助项目(30800325) |
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| 摘 要： | 目的:检测和比较6种针对大鼠Nogo-A分子不同表位的单克隆抗体(mAb)应用于中枢神经组织的免疫组化染色特性,从而决定它们今后可能的应用范围和方法。其中4种mAb是针对Nogo-A分子中Nogo66片段,分别命名为No-go66-1、Nogo66-2、Nogo66-3和Nogo66-4;其他2种mAb是针对Nogo-A分子氨基端(570-691)肽段,分别为Nogo-N1和Nogo-N2。方法:利用免疫荧光组织化学,检测Nogo-A mAb抗体对大鼠神经组织的反应性与特异性。结果:正常SD大鼠脊髓组织的免疫荧光组织化学双标结果显示,制备的6种抗大鼠Nogo-A分子mAb均可以与商品化的兔抗大鼠Nogo-A多克隆抗体(PcAb)双标记;其中Nogo66-1、Nogo66-2、No-go66-4和NogoN-2 mAb可与MBP阳性细胞双标记,与GFAP阳性细胞不共存;而Nogo66-3和NogoN-1 mAb不仅可以与MBP阳性细胞双标,同时也可以和GFAP阳性细胞共存。结论:Nogo66-1、Nogo66-2、Nogo66-4和NogoN-2 mAb可很好的识别组织中的Nogo-A分子,用于免疫组化研究。
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| 关 键 词： | Nogo-A 单克隆抗体 免疫荧光 组织化学 |
Identification of six kinds of monoclonal antibodies against rat Nogo-A molecule in the immunofluorescent histochemical staining |
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| DENG Bin,LIU Fang-fang,ZHAO Xiang-hui,YU Cai-yong,BIAN Gan-lan,FENG Rui,JU Gong,WANG Jian.Identification of six kinds of monoclonal antibodies against rat Nogo-A molecule in the immunofluorescent histochemical staining[J].Journal of Cellular and Molecular Immunology,2011,27(4):425-427. |
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| Authors: | DENG Bin LIU Fang-fang ZHAO Xiang-hui YU Cai-yong BIAN Gan-lan FENG Rui JU Gong WANG Jian |
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| Institution: | DENG Bin,LIU Fang-fang,ZHAO Xiang-hui,YU Cai-yong,BIAN Gan-lan,FENG Rui,JU Gong,WANG Jian Department of Neurobiology,Fourth Military Medical University,Xi'an 710032,China |
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| Abstract: | AIM:To detect and characterize of 6 monoclonal antibodies(mAbs) against different epitopes of rat Nogo-A molecule in immunohistochemistry to decide their applications in futrue.Four mAbs against Nogo66 fragment are named Nogo66-1,Nogo66-2,Nogo66-3 and Nogo66-4.The rest of 2 mAbs against N-termial 570-691aa fragment are named NogoN-1 and NogoN-2.METHODS: The immunofluorescence staining was used to detect the reactivity and specificity of those 6 mAbs in spinal tissue sections of rat.RESULTS: All 6 mAbs were ... |
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| Keywords: | Nogo-A monoclonal antibody immunofluorescence histochemistry |
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