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| 优化附子生物碱提取工艺的实验研究 | |
| 引用本文： | 曾亦斌,张宇燕,杨洁红,万海同,李金辉. 优化附子生物碱提取工艺的实验研究[J]. 中国中医急症, 2011, 20(3): 413-414 |
| 作者姓名： | 曾亦斌 张宇燕 杨洁红 万海同 李金辉 |
| 作者单位： | 浙江中医药大学,浙江杭州,310053 |
| 基金项目： | 浙江省中医药管理局，浙江省教育厅项目 |
| 摘 要： | 目的优化附子生物碱提取的工艺。方法采用紫外-可见分光光度法建立乌头碱含量的测定方法;考察不同提取溶剂对提取率的影响,通过正交实验考察提取时间、溶剂质量浓度、溶剂用量等因素进一步优化工艺条件。结果乌头碱的线性回归方程为：y=2.8819x-0.0019（r2=0.9991）,乌头碱在0.025～0.255mg/mL范围内呈良好的线性关系。经过正交设计研究并通过工艺验证,乌头碱的最佳提取工艺条件为10倍量1.5mol/L的盐酸溶液煎煮3h。结论优化的提取工艺可为含乌头碱类成分的药材提取及制剂的制备提供参考。 |
| 关 键 词： | 附子 乌头碱 提取 工艺条件 优化 |
Study on Optimizing the Extraction Process of Alkaloids from Aconite |
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| ZENG Yi-bin,ZHANG Yu-yan,YANG Jie-hong,et al Zhejiang Chinese Medical University. Study on Optimizing the Extraction Process of Alkaloids from Aconite[J]. Journal of Emergency in Traditional Chinese Medicine, 2011, 20(3): 413-414 | |
| Authors: | ZENG Yi-bin ZHANG Yu-yan YANG Jie-hong et al Zhejiang Chinese Medical University |
| Affiliation: | ZENG Yi-bin,ZHANG Yu-yan,YANG Jie-hong,et al Zhejiang Chinese Medical University(Hangzhou,Zhejiang 310053) |
| Abstract: | Objective:To optimizate the extraction precess of alkaloid in Radix Aconiti Lateralis Preparata.Methods:The assay method was established by ultraviolet-visible spectrophotometry on the content of aconitine.The extraction rate of different extraction solvents was investigated and optimizited different factors such as extracting time,solvent quality concentration and solvent usage amount on the conditions of extracting process by orthogonal design.Results:Linear regression equation of aconitine:y=2.8819x-0.00... |
| Keywords: | Radix Aconiti Lateralis Preparata Aconitine Extraction Process conditions Optimization |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |