建立卵巢过度刺激综合征大鼠模型的研究

目的构建卵巢过度刺激综合征(OHSS)的大鼠模型。方法32只未成年Wistar雌鼠,随机分为常规刺激组和过度刺激组。过度刺激组:22日龄Wistar雌鼠连续4天皮下注射含有10 IU PMSG的生理盐水0.2ml,至26日龄皮下注射含有30 IU hCG的生理盐水0.2ml。设立对照组为常规刺激组:24日龄Wistar雌鼠皮下注射含有10IU PMSG的生理盐水0.2ml,再予皮下注射含有10IU hCG的生理盐水0.2ml。测血E2水平,尾静脉注入美兰溶液30min后取腹腔灌洗液和卵巢,用分光光度计分别测定腹腔灌洗液和卵巢组织中的美兰含量来判断腹膜和卵巢血管通透性;比较两组间卵巢重量,血E2水平及卵巢、腹腔液中美兰含量。结果注射hCG 48h后,过度刺激组大鼠卵巢重量、血E2水平、腹腔液和卵巢中美兰含量均显著高于常规刺激组。结论利用适当日龄Wister大鼠可以成功构建OHSS动物模型。

Study on Establishment of the Animal Model of Ovarian Hyperstimulation Syndrome

Objective To establish the rat model of ovarian hyperstimulation syndrome(OHSS).Methods Immature female Wistar rats aged 22 days were divided into two groups: the OHSS group(16 rats) was given 10 IU PMSG for 4 consecutive days and given 30 IU hCG on 26-day-old to produce ovarian hyperstimulated manifestations;the conventional stimulation group(16 rats) was given 10 IU PMSG on 24-day-old and given 10 IU hCG on 26-day-old.On 28-day-old,the rats were injected via the tail vein Methylene blue dye solution to evaluate the capillary permeability in the peritoneal cavity and ovary;blood samples were collected from the inferior caval vein to evaluate serum estradiol(E2).Results Compared with the conventional group,the contents of methylene blue in peritoneal cavity and ovarian were significantly higher in the OHSS group;Ovarian wet weights and serum concentration of E2 were also markedly increased.Conclusion The animal model of OHSS can be induced by injected PMSG and hCG on immature female Wistar rats.