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以双向凝胶电泳为基础的食管癌组织蛋白质组学分析新策略
引用本文:王立顺,尹艳慧,高素君,殷剑宁,赵晓航,朱迅. 以双向凝胶电泳为基础的食管癌组织蛋白质组学分析新策略[J]. 中国免疫学杂志, 2003, 19(4): 228-231
作者姓名:王立顺  尹艳慧  高素君  殷剑宁  赵晓航  朱迅
作者单位:1. 吉林大学基础医学院免疫教研室,长春,130021
2. 吉林大学第一临床医学院血液科,长春,130021
3. 北京华大基因研究中心,北京,100000
4. 中国医学科学院肿瘤研究所,北京,100034
摘    要:
目的:探讨采用双向电泳为基础的蛋白质组学的研究手段在食管癌组织与食管癌旁组织差异分析中的应用。方法:双向凝胶电泳分离食管癌及其癌旁组织的蛋白质,图像分析软件ImageMaster4.01分析所获得的凝胶图谱并寻找差异点,用质谱仪鉴定差异点蛋白质。结果:提出了一套可行的癌组织差异分析策略,并发现在分子量50 000-60 000,等电点5—6的区域,食管癌组织和食管癌旁组织存在2个差异表达的蛋白,质谱鉴定为丙酮酸激酶M2/M1。结论:在合理的策略指导下,以双向凝胶电泳为基础的蛋白质组学技术是研究肿瘤的一种重要手段,我们得到的丙酮酸激酶M2/M1在食管癌与其癌旁组织中存在差异表达,可能对食管癌的诊断、治疗具有重要意义。

关 键 词:双向凝胶电泳 食管癌组织蛋 白质组学 分析
文章编号:1000-484X(2003)04-0228-04

New strategy of two-dimensional gel electrophoresis-based proteomics analysis in esophageal cancer
WANG Li Shun,YIN Yan Hui,GAO Su Jun et al. New strategy of two-dimensional gel electrophoresis-based proteomics analysis in esophageal cancer[J]. Chinese Journal of Immunology, 2003, 19(4): 228-231
Authors:WANG Li Shun  YIN Yan Hui  GAO Su Jun et al
Affiliation:WANG Li Shun,YIN Yan Hui,GAO Su Jun et al. Department of Immunology,School of Basic Medical Sciences,Jilin University,Changchun 130021,China
Abstract:
Objective:To apply the strategy of proteomics with two dimensional gel electrophoresis(2DE) in esophageal cancer.Methods:the protein of esophageal cancer tissues and its adjacent normal tissue were separated by 2DE, the gels were analyzed with software ImageMaster4.01 to find out the spots different between cancer and control, and the proteins in the spots were identified by matrix associated laser desorption/ionization time of flight(MALDI TOF) mass spectrometry.Results:a new reasonable strategy of study for proteomics on esophageal cancer was raised and candidate cancer marker of two pyruvate kinase isozyme were found under such strategy.Conclusion:Under reasonable strategy, the analysis of proteomics with 2DE on human tissue is a useful method for discovering valuable cancer marker candidates. Pyruvate kinase isozyme M2/M1 which were found in cancer tussues will be very important in diagnosis and treatment of esophageal cancer.
Keywords:Proteomics  Esophageal cancer  2DE  
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