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| Apoptin对肝癌细胞的体内、外抑制效应 | |
| 引用本文： | 刘立明,金宁一,李 霄,田明尧,杨恩成,刘 妍,赵翠青,张金双,钱爱东. Apoptin对肝癌细胞的体内、外抑制效应[J]. 中国肿瘤生物治疗杂志, 2009, 16(2): 161-164. DOI: 10.3872/j.issn.1007-385X.2009.02.012 |
| 作者姓名： | 刘立明 金宁一 李 霄 田明尧 杨恩成 刘 妍 赵翠青 张金双 钱爱东 |
| 作者单位： | 1. 军事医学科学院,全军基因工程实验室,吉林,长春,130062;吉林农业大学,动物科学技术学院,吉林,长春,130118 2. 军事医学科学院,全军基因工程实验室,吉林,长春,130062 3. 军事医学科学院,全军基因工程实验室,吉林,长春,130062;吉林大学第一临床医院,吉林,长春,130021;黑龙江省医院消化病院,黑龙江,哈尔滨,150001 4. 军事医学科学院,全军基因工程实验室,吉林,长春,130062;吉林大学第一临床医院,吉林,长春,130021 5. 吉林农业大学,动物科学技术学院,吉林,长春,130118 |
| 基金项目： | 国家高技术研究发展计划(863)资助项目(No. 2007AA021004) |
| 摘 要： | 目的: 探讨Apoptin对人肝癌细胞株HepG2及C57BL/6小鼠H22移植瘤的抑制作用。方法：应用脂质体转染法将重组质粒pVAX1Apoptin转染HepG2细胞，应用Western blotting检测Apoptin蛋白在转染后HepG2细胞中的表达，应用MTT检测Apoptin对HepG2细胞生长的抑制作用，通过AO/EB染色法检测pVAX1Apoptin致肿瘤细胞凋亡作用。建立C57BL/6小鼠H22荷瘤模型，瘤内注射pVAX1Apoptin，观察pVAX1Apoptin对体内肿瘤的抑制作用。结果： Apoptin基因可在HepG2细胞中有效表达，pVAX1Apoptin能够诱导HepG2细胞凋亡，并显著地抑制其生长，48 h抑制率为69.28%。pVAX1Apoptin瘤内注射能够有效抑制移植肿瘤的生长，抑制率为46.71%；治疗后39 d小鼠生存率为40%，显著高于对照组(P<005)。结论： Apoptin转染可抑制HepG2细胞的生长，重组质粒pVAX1Apoptin瘤内注射对移植肿瘤有显著的抑制作用。 |
| 关 键 词： | 肝肿瘤 H22荷瘤模型 增殖 凋亡 |
| 收稿时间： | 2009-01-19 |
| 修稿时间： | 2009-02-11 |
Anti-tumor effects of Apoptin gene against hepatoma cells in vivo and in vitro |
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| LIU Li ming,JIN Ning yi,LI Xiao,TIAN Ming yao,YANG En cheng,LIU Yan,ZHAO Cui qing,ZHANG Jin shuang and QIAN Ai dong. Anti-tumor effects of Apoptin gene against hepatoma cells in vivo and in vitro[J]. Chinses Journal of Cancer Biotherapy, 2009, 16(2): 161-164. DOI: 10.3872/j.issn.1007-385X.2009.02.012 | |
| Authors: | LIU Li ming JIN Ning yi LI Xiao TIAN Ming yao YANG En cheng LIU Yan ZHAO Cui qing ZHANG Jin shuang QIAN Ai dong |
| Affiliation: | Key Laboratory of Genetic Engineering of PLA, Academy of Military Medical Sciences, Changchun 130062, Jilin, China; College of Animal Science and Technology, Jilin Agriculture University, Changchun 130118, Jilin, China;Key Laboratory of Genetic Engineering of PLA, Academy of Military Medical Sciences, Changchun 130062, Jilin, China;Key Laboratory of Genetic Engineering of PLA, Academy of Military Medical Sciences, Changchun 130062, Jilin, China;Key Laboratory of Genetic Engineering of PLA, Academy of Military Medical Sciences, Changchun 130062, Jilin, China;Key Laboratory of Genetic Engineering of PLA, Academy of Military Medical Sciences, Changchun 130062, Jilin, China; First Clinical Hospital, Jilin University, Changchun 130021, Jilin, China;Department of Digestive Diseases, Heilongjiang Provincial Hospita;Key Laboratory of Genetic Engineering of PLA, Academy of Military Medical Sciences, Changchun 130062, Jilin, China;First Clinical Hospital, Jilin University, Changchun 130021, Jilin, China;Key Laboratory of Genetic Engineering of PLA, Academy of Military Medical Sciences, Changchun 130062, Jilin, China;Key Laboratory of Genetic Engineering of PLA, Academy of Military Medical Sciences, Changchun 130062, Jilin, China;College of Animal Science and Technology, Jilin Agriculture University, Changchun 130118, Jilin, China |
| Abstract: | |
| Keywords: | Apoptin HepG-2 |
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