宁夏枸杞根际土壤链霉菌V-1-3次级代谢产物分析

摘要：目的 获得链霉菌V-1-3的基因组序列信息，分析其次级代谢产物生物合成基因簇并预测其代谢产物，为发现潜在新抗生素奠定基础。方法 基于16S rRNA基因序列进行菌株属水平鉴定，利用Illumina HiSeq+PacBio测序技术对菌株V-1-3进行基因组测序，采用antiSMASH(v6.0.1)在线工具分析次级代谢产物生物合成基因簇，液-质联用技术检测产生的次级代谢产物。结果 链霉菌V-1-3基因组序列全长8 243 417 bp，平均(G+C)含量为72.14 %，共编码7578个基因，预测到33个生物合成基因簇，利用液-质联用检测到4个代谢物：oxalomycin B、geosmin、coelichelin和ishigamide。结论 来自盐碱地的链霉菌V-1-3具有丰富的次级代谢产物生物合成基因簇，能产生多种次级代谢产物，具有进一步发掘新抗生素的价值。

Study on secondary metabolites of Streptomyces sp. V-1-3 from the rhizosphere soil of Lycium barbarum in Ningxia

Abstract Objective To mine secondary metabolite biosynthesis gene clusters of Streptomyces sp. V-1-3, predict its metabolites and lay the foundation for the discovery of new antibiotics. Methods The 16S rRNA gene sequencing was employed to preliminarily identify the strain. Streptomyces sp. V-1-3 was sequenced by Illumina HiSeq + PacBio sequencing technology to mine secondary metabolite by antiSMASH (v6.0.1) online tool and the secondary metabolites of Streptomyces sp. V-1-3 were further analyzed by HPLC-Q-TOF MS. Results The size of the genome was 8,243 417 bp with an average of G+C content at 72.14 %, which had 7,578 genes. Biosynthetic gene clusters for thirty-three secondary metabolites were predicted. Four compounds, oxalomycin B, geosmin, coelichelin, and ishigamide were validated by HPLC-Q-TOF MS analysis. Conclusion Streptomyces sp. V-1-3 from saline-alkali soil has abundant secondary metabolite biosynthesis gene clusters, and can produce different types of secondary metabolites, which has the value for further exploring to find new