白藜芦醇联合γ射线调控PI3K/Akt/mTOR信号通路对宫颈癌Hela细胞的影响

目的 探讨白藜芦醇联合γ射线对宫颈癌Hela细胞生物学行为的影响,并对其可能机制进行初步探究。方法 CCK-8法检测不同浓度白藜芦醇溶液 ±γ射线照射后细胞群体增殖;划痕实验和Transwell小室实验检测细胞迁移和侵袭;流式细胞术和Annexin V-FITC/PI双染检测细胞凋亡;蛋白印迹法检测PI3K、Akt、p-Akt、mTOR、p-mTOR蛋白表达。结果 与对照组相比,白藜芦醇组 ±γ射线照射均能抑制细胞增殖、迁移、侵袭能力,并促进细胞凋亡,且联合的效果更加明显。与对照组相比,白藜芦醇和γ射线均能降低细胞中Bcl-2、PI3K、p-Akt、p-mTOR蛋白表达,使Bax蛋白表达上调,但Akt、mTOR蛋白未发生明显改变。结论 白藜芦醇联合γ射线能够明显抑制Hela细胞增殖、迁移、侵袭能力并促进细胞凋亡,其机制可能与PI3K/Akt/mTOR信号通路的抑制作用及下游相关蛋白的表达相关。

Effect of resveratrol combined with radiotherapy on cervical cancer Hela cell growth and migration by regulating PI3K/Akt/mTOR signaling pathway

Objective To evaluate the effect of resveratrol combined with γ-ray irradiation on the biological behavior of cervical cancer cells, and to explore its possible mechanism. Methods The proliferation of cell populations after different concentrations of resveratrol solution±γ-ray irradiation was detected by CCK-8 assay. Scratch test and Transwell chamber test were used to detect cell migration and invasion. Flow cytometry and Annexin V-FITC/PI double staining were employed to assess cell apoptosis. Western blot was performed to measure the expression levels of PI3K, Akt, p-Akt, mTOR and p-mTOR proteins. Results Compared with the normal control (NC) group, the resveratrol group±γ-ray irradiation could inhibit the proliferation, migration, and invasion and promote cell apoptosis of human cervical cancer Hela cells, and the combined effect was more obvious. Compared with the NC group, resveratrol and γ-ray irradiation could significantly down-regulate the expression levels of Bcl-2, PI3K, p-Akt and p-mTOR proteins, up-regulate the expression level of Bax protein, but did not significantly alter the expression levels of Akt and mTOR proteins in human cervic1255al cancer Hela cells. Conclusions Resveratrol combined with γ-ray irradiation can dramatically inhibit the proliferation, migration, invasion, and promote the apoptosis of cervical cancer Hela cells. The mechanism may be related to the inhibition of the PI3K/Akt/mTOR signaling pathway and down-regulating the expression levels of downstream related proteins.