丹参对乳鼠心肌细胞凋亡的影响

目的 :观察丹参对乳鼠心肌细胞凋亡的影响 ,并探索其作用机制。方法 :体外培养的 Wistar乳鼠心肌细胞缺糖缺氧 6 h后 ,采用心肌酶检测其代谢及流式细胞术检测心肌细胞凋亡。结果 :缺糖缺氧组心肌细胞凋亡数量及酶指数显著高于正常对照组 ,大、小剂量丹参液组心肌细胞凋亡及酶指数明显下降 ,与缺糖缺氧组比较有显著性差异。结论 :丹参对缺糖缺氧心肌细胞凋亡有明显的抑制作用

Effects of Salvia Miltiorrhiza Bunge on cardiomyocyte apoptosis in neonatal rats

To investi gate the effect and the mechanism of Salvia Mil tiorrhiza Injection(SMI) on cardiomyocy tes apoptosis,neonatal Wistar rat cardiomyocytes were cultured under hypoxicThe apoptotic myocytes an d myocardio-enzyme method wed used to detect the change of myocytes metabolismΤhe results showed that the numbe rs of apoptotic cell and myocardio -enzyme in the hypoxic in the glucose deprivation group were greater th an those in the control group respe ctively;the number of apoptotic ce lls and the level of myocardio-enz yme of the two Salvia Miltorrhiza Bunge groups were less than those in the hypoxic group respectively,bu t similar to those in the control groupThe se results indicate that apoptosis is an important mechanism of myoc yte cell death under the condition of hypoxia and glucose deprivatio n and that SMI can suppressed the apoptosis greatly