氧自由基诱发离体大鼠胃粘膜细胞的损伤

本实验采用Pronase-EDTA法分离大鼠胃粘膜细胞,以黄嘌氧化酶(XO)-黄嘌呤(X)系统产生氧自由基,观察了氧自由基对离体孵育的胃粘膜细胞的损伤作用。结果表明,XO—X系统可使细胞存活率下降和乳酸脱氢酶(LDH)漏出增加,二者呈显著的负相关关系,其变化幅度与XO—X的作用时间或XO浓度呈依从关系;O_2清除剂超氧化物歧化酶和OH清除剂甘露醇及二甲亚砜均可减轻XO—X诱发的细胞存活率下降及LDH漏出增加。表明,O_2和OH在XO—X诱发的离体胃粘膜细胞损伤中起主要作用。

OXYGEN RADICAL-INDUCED INJURY OF ISOLATED GASTRIC MUCOSAL CELLS OF THE RAT

The present work observed the role of oxygen radicals on the injury of gastric mucosal cells whichwere isolated from the rat with pronase-EDTA method. Oxygen radicals were produced by xanthineoxidase (XO)-xanthine (X) system. The results showed, that XO-X system could reduce the viabilityof the cells and increase the lactate dehydrogenase (LDH) leakage from the cells, that the variationsof both had a negative correlationship and were dependent on the duration of XO-X acting on thecells and on the concentration of XO, and that superoxide dismutase (a scavenger of superoxide radi-cal) and mannitol or dimethyl sulfoxide (two scavengers of hydroxy radical) might inhibit the reduc-tion of cell viability and the LDH leakage from the cells induced with XO-X system. The above resultssuggested that superoxide and hydroxy radicals played a main role in the injury of isolated gastricmucosal cells induced with XO-X system.