药物干预对哮喘小鼠模型Th1/Th2细胞因子表达的影响

目的 探讨胸腺素-α和地塞米松对辅助型T淋巴细胞1型/辅助型T淋巴细胞2型（Th1/Th2）细胞因子在哮喘发病过程中的干预作用及其临床应用价值。方法 将小鼠分为对照组、哮喘模型组、胸腺素干预组、地塞米松干预组。采用酶联免疫吸附试验（ELISA）法,测定支气管肺泡灌洗液（BALF）中白细胞介素-4（IL-4）、白细胞介素-5（IL-5）和干扰素 γ（IFN γ）的水平。结果 与对照组相比,哮喘组的嗜酸性粒细胞（EOS）、淋巴细胞、IL 4、IL 5升高, IFN γ下降（P<0.05）。与哮喘组相比,胸腺素组和地塞米松组的EOS、IL-4、IL-5减少,胸腺素组IFN-γ增加（P<0.05）。对照组与哮喘组、哮喘组与胸腺素组、哮喘组与地塞米松组之间的IL 4/IFN γ相比,差异均有统计学意义（P均<0.05）。结论 Th2优势反应在哮喘的发病机制中起重要作用,胸腺素-α可明显提高IFN-γ、Th1的水平,地塞米松可以降低IL-4、IL-5及Th2水平。

Interfering effects of thymosine and dexamethasone on TH1/TH2 cytokines in the asthma mouse model

Objective    To explore the role of Th1/Th2 cytokines in the pathogenic process of asthma,interfering effects of thymosine-α and dexamethasone-on Th1/Th2 and their clinical values. Methods     An asthma model was set up and thymosine-α and dexamethasone were given. The rats were divided into the normal control group,the asthmic group,the thymosin group and the dexamethasone group.Levels of IL-4, IL-5 and IFN-γin bronchoalveolar lavage fluid(BALF) were detected by ELISA. Results     There was a significant increase in the numbers of EOS and lymphocytes and levels of IL-4 and IL-5, while there was a significant decrease in the level of IFN-γ in the asthmic group compared with the normal control group (P<0.05). Number of EOS and levels of IL-4 and IL-5 decreased in the thymosine dexamethasone groups compared with the asthmic group, and the difference was significant (P<0.05). The level of IFN-γ in the thymosin group increased compared with the asthmic group and the difference was significant(P<0.05). For IL-4/IFN-γ, there were notable differences between the normal group and the asthmic  group,between the asthmic group and the thymosin group,and between the asthmic group and the dexamethasone group(P<0.05). Conclusion    The Th2 dominant reaction play an important role in the pathogenesis of asthma.Thymosin-α can notably increase the level of IFN-γ. Dexamethasone can lower the levels of IL-4 and IL-5.