Comparison of Different Assays To Assess Human Papillomavirus (HPV) Type 16- and 18-Specific Antibodies after HPV Infection and Vaccination |
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| Authors: | Mirte Scherpenisse Rutger M. Schepp Madelief Mollers Sofie H. Mooij Chris J. L. M. Meijer Guy A. M. Berbers Fiona R. M. van der Klis |
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| Affiliation: | Laboratory for Infectious Diseases and Screening, National Institute of Public Health and the Environment, Bilthoven, the Netherlandsa;Department of Epidemiology and Surveillance, National Institute of Public Health and the Environment, Bilthoven, the Netherlandsb;Department of Pathology, VU University Medical Center, Amsterdam, the Netherlandsc;Public Health Service of Amsterdam (GGD), Amsterdam, the Netherlandsd |
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| Abstract: | We compared the measurement of human papillomavirus (HPV)-specific serum antibody levels with the virus-like-particle multiplex immunoassay (VLP-MIA), competitive Luminex immunoassay (cLIA), and glutathione S-transferase (GST) L1-based MIA. Using a large panel of serum samples, these assays showed mutually good correlations for both naturally induced and vaccine-derived HPV-specific antibody levels. However, an adaptation of the GST L1-based MIA resulted in an improved correlation with both cLIA and VLP-MIA. |
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