脑胶质瘤SHG-44细胞动力学特征的实验研究

目的　探讨脑胶质瘤潜在倍增时间 (Tpot)实验条件。方法　采用荷脑瘤动物模型 ,溴脱氧尿嘧啶核苷(BudR)掺入法测定Tpot。结果　所建立的Wistar脑胶质瘤大鼠模型实用、可靠。在体潜在倍增时间、有效倍增时间 (Teff)分别为 8.15天和 18.4 6天。BudR掺入率、细胞标记指数 (LI)均在BudR掺入后 8h达最高 ,>6Gy辐射其潜在倍增时间明显缩短。结论　在体、离体BudR掺入标记率存在明显差异。辐射吸收剂量 >12Gy,其实际倍增时间、潜在倍增时间、有效倍增时间降至最低点。提示脑胶质瘤放疗过程中存在细胞加速再增殖 ,宜连续治疗

The dynamic characterization of glioma cell line SHG-44

Objective To study the factors regulating the potential doubling time (T pot ) in experimental rat cerebral glioma.?Methods The cerebral glioma model was built by transplanting SHG-44 cells into the brain of Winster rat, and the T pot and time of DNA synthesis (Ts) were measured by BudR incorporation trials.?Results The established glioma rat model served quite well in the experiment. The T pot and the effective doubling time (T eff ) in vivo were 8.15 days and 18.4 days respectively. There were significant differences between in vivo and in vitro BudR trials. When BudR had been incorporated for 8 hours, the label index on SHG-44 cells was the highest. After radiotherapy given was more than 6 Gy, the T pot was shortened significasntly. And when the radiotherapy given was added to more than 12 Gy, the actual doubling time, T pot and T eff would decline to the lowest point.?Conclusion The glioma cells will repopulate rapidly when the radiotherapy is started. Hence, the radiotherapy should be continued step by step in the routine manner.