Cytogenetic studies in permissive and abortive infections by adenovirus type 12

Chromosome re-arrangements have been found in human cells infected in vitro with adenovirus type 12. Dicentric and ring chromosomes and chromatid re-arrangements were induced in cells infected during transition from GI to S phase. In non-permissive hybrid cells the development of monoclonal stem lines, associated with the persistence of the viral genome, has been demonstrated. Persistence of the virus in these cells results in continued induction of the uncoiler region of human chromosome E17. The association of viral nucleic acid with host-cell chromosomes has been confirmed by the in situ hybridization method.