硫酸镁对急性颅脑损伤一氧化氮合成酶活性的影响

目的：观察硫酸镁对急性颅脑损伤脑组织一氧化氮合成酶（Nitric oxide syantrase,NOS)活性的影响，探讨镁离子对急性颅脑损伤的治疗作用及机理。方法：建立大鼠脑外伤模型，采用化学定量法对大鼠脑外伤后脑组织中NOS含量进行检测，结果：大鼠脑皮质中的NOS活性在 后30min较对照组升高（P＜0．05），2h后较对照组升高非常显著（P＜0．01），6h开始下降，24h降至基础水平，硫酸镁治疗组在伤后2h,6h,NOS活性较损伤组明显降低（P＜0．01，P＜0．05），结论：颅脑损伤后，受损脑组织中NOS活性升,以酸镁可通过抑制损伤后NOS活性，起到保护创伤神经元的作用。

Experimental study of influence of magnesium sulfate on NOS activity for acute craniocerebral injury

Objective:To observe the influence of magnesium sulfate on nitric oxide syantrase(NOS)activity in acute craniocerebral injury tissue and discuss the treatment action and mechanism of magnesium ion for acute craniocerebral injury.Methods:Brain trauma model of rats was established,and chemical quantitative method was used to test the content of NOS in rat brain tissue after its brain was injured.Results:NOS activity in rat cerebral cortes was higher than that in the contrasting group post-traumatic 30 min (P<0.05),significantly higher than that in the contrasting group post-traumatic 2h(P<0.01),and NOS activity began to reduce post-traumatic 6h and dropped to the basic level post-traumatic 24h.NOS activity in magnesium sulfate treatment group was obviously lower than that in the injured group post-traumatic 2h and 6h(P<0.01,P<0.05).Conclusion:After craniocerebral was injured,NOS activity in the injured cerebral tissue increased,and magnesium sulfate could take effect in defending neuron by inhibiting NOS activity after injury.