竹红菌乙素脂质体对鸡冠皮肤光动力效应的初步研究

目的　观察竹红菌乙素脂质体 (HB liposome)光动力学疗法对鸡冠皮肤的光敏损伤特点。方法　成年来亨鸡 4 5只 ,随机分为对照组 9只 ,PDT组 36只。PDT组动物静脉分别注射HB liposome 0 2 5、0 5、0 75mg/kg ,给药后即刻用铜蒸气激光照射 ,功率密度分别为 5 0、1 0 0mW/cm2 ,能量密度分别为 30、6 0、1 2 0J/cm2 ,于照射后第 1、3、7、1 4、2 8天进行肉眼和光镜观察。结果　光敏剂剂量为 0 2 5、0 5mg/kg时 ,以功率密度为 1 0 0mW /cm2 的铜蒸气激光混合光照射 2 0min ,肉眼和光镜观察显示来亨鸡鸡冠的真皮乳头层毛细血管网破坏明显 ,表皮层无明显损伤 ,褪色效果明显。结论　HB liposome光动力学疗法对鲜红斑痣模型—来亨鸡鸡冠有着良好的褪色效果。

An Experimental Study of Photodynamic Effect of Hypocrelline B Liposome on Leghorn Cock Comb

Objective To investigate HB-liposome photodynamic effect on port wine stain(PWS) and to understand its injury character by using leghorn cock comb as an animal model through naked eye and light-microscopy observation. Methods Among 45 adult Leghorn cock with undamaged combs, divided into a control group and experiment group at random. Experiment group was injected HB-liposome at 0.25,0.5,0.75 mg/kg dose and irradiated by copper vapor laser immediately. The power density is 50,100 mW/cm 2 and the energy density is 30,60 and 120 J/cm 2. The eye observation and light microscopy were concluded on the combs 1,3,7,14 and 28 days after treatment. Results Results demonstrated that the red combs faded completely without damaging of the overlying skin. HB-liposome-PDT has good depigmentation effect to PWS`s animal model-leghorn cock comb at dose of 0.25 and 0.5mg/kg and in combination with 20 min irradiation of 100 mW/cm2 of copper vapor laser through naked eye and light microscopy observation. Conclusions HB-liposome-PDT has good depigmentation effect on PWS `s animal model-leghorn cock comb at suitable drug and light dose.