| 槲皮素诱导人白血病HL—60细胞凋亡 |
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| 引用本文： | 肖东 朱寿彭. 槲皮素诱导人白血病HL—60细胞凋亡[J]. 中国药理学报, 1997, 18(3): 280-283 |
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| 作者姓名： | 肖东 朱寿彭 |
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| 作者单位： | [1]苏州医学院药理学教研室 [2]放射毒理学教研室 |
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| 摘 要： | ![]()
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| 关 键 词： | 槲皮素 细胞凋亡 HL－60细胞 白血病 |
Quercetin induced apoptosis in human leukemia HL-60 cells. |
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| D Xiao,S P Zhu,Z L Gu. Quercetin induced apoptosis in human leukemia HL-60 cells.[J]. Acta Pharmacologica Sinica, 1997, 18(3): 280-283 |
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| Authors: | D Xiao S P Zhu Z L Gu |
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| Affiliation: | Department of Pharmacology, Suzhou Medical College, China. |
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| Abstract: | ![]()
AIM: To examine whether quercetin (Que) might induce apoptosis in human leukemia HL-60 cells. METHODS: DNA fragmentation was visualized by agarose gel electrophoresis. Inhibition of proliferation was measured with a colorimetric MTT-assay. The DNA degradation was determined using flow cytometry, and the microscopic changes were observed by an electron microscope. RESULTS: Que 15-120 mumol.L-1 elicited typical apoptosis morphological changes including condensed chromatin, nuclear fragmentation, and reduction in volume. DNA fragmentation and DNA degradation in a concentration-dependent manner in HL-60 cells. Que inhibited HL-60 cell proliferation. The values of IC50 and 95% confidence limits were 43 (30-61) mumol.L-1 after 48-h treatment with Que. CONCLUSION: Que induced apoptosis in HL-60 cells. |
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