隔离饲养及给药时刻对小鼠阿米卡星药物动力学的影响

目的：研究社会环境及给药时刻对小鼠阿米卡星代谢的影响。方法：小鼠按饲养环境；隔离饲养（Ｉ）或集体饲养（Ａ）及给药时间：日中（Ｄ）及午液（Ｎ）随机分为：Ｉ－Ｄ，Ｉ－Ｎ，Ａ－Ｄ，Ａ－Ｎ４组，饲养４周后于Ｄ（１３：００）或Ｎ（０１：００）ｓｃ阿米卡星１５ｍｇ．ｋｇ＾－１测定给药后血浆浓度，以开放一室模型拟合计算有关药代动力学参数，结果：Ａ－Ｎ组阿米卡星清除较率Ａ－Ｄ及Ｉ－Ｎ组增大，血浆半衰期变短，０－１

Effects of isolation housing and timing of drug administration on amikacin kinetics in mice.

AIM: To study the influences of social condition and drug administration time on amikacin metabolism in mice. METHODS: Forty Male ICR mice were randomly assigned into 4 groups according to 1) housing condition: individual housing (I, one mouse in a cage) or aggregated housing (A, 10 mice in a cage) and 2) drug administration time: at midday (D) or at midnight (N), i.e. I-D, I-N, A-D, and A-N groups. Amikacin was injected s.c. 15 mg.kg-1 after 4 wk of raising at D or N. Blood samples were taken at 5, 10, 15, 20, 30, and 60 min after medication in each mouse. Plasma amikacin was measured by enzyme immunoassay. The concentration-time data were fitted with one-compartment open model in each mouse and data were analyzed with group t test. RESULTS: The clearance (Cl) of amikacin was larger and the half-life (T1/2) was shorter in A-N group than in A-D or I-N groups respectively. AUC(0-1) in A-N group was less than in I-N group. No differences of kinetic parameters between 2 isolated housing (I-D and I-N) groups were found. CONCLUSION: Aggregated housing and midnight drug administration increased the disposition of amikacin.