双向发酵对川乌指纹图谱及乌头碱类成分含量的影响

目的:观察川乌被19种药用真菌发酵后,乌头碱、新乌头碱、次乌头碱含量的变化及高效液相色谱(HPLC)指纹图谱的差异。方法:将川乌作为"药性基质",在一定的条件控制下,应用双向发酵技术,使其被槐耳、灵芝、红栓等19种药用真菌发酵,产生药材的"药性菌质",并采用反相高效液相色谱法对"药性菌质"和生药材中所含的乌头碱、新乌头碱和次乌头碱进行含量测定,比较其含量的变化及指纹图谱的差异。结果:在一定的时间范围内,大多数的菌株在川乌基质上的适应性良好,菌丝体生长旺盛,如代号A、C、D、E、F、M、Q、V、SW、い等真菌。发酵之后的川乌中乌头碱、新乌头碱及次乌头碱含量与生药材比较多数有明显的降低。结论:双向发酵后多数"药性菌质"中乌头碱、新乌头碱和次乌头碱含量明显降低了,提示双向发酵是生物技术在中医药领域中应用的有益尝试。

Effects of Two-way Fermentation on the Fingerprints of Aconitum carmichaeli and the Content of Aconitine

OBJECTIVE：To observe the contents of aconitine,mesaconine and hypaconitine and the difference of HPLC fingerprints of Aconitum carmichaeli after fermented with 19 kinds of medicine fungus.METHODS：Under the control of certain experiment conditions,A.carmichaeli was used as ground substance and fermented by two-way fermentation with 19 kinds of medicine fungus,such as Trametes robiniophila,Ganoderma lucidum,Pycnoporus cinnabarius.The product of medicinal ground substance mixed with mycelia was called ＂medicinal mycoplasma＂.An RP-HPLC method was then established to determine the contents of aconitine,mesaconine and hypaconitine.The difference of the content and HPLC fingerprints were compared.RESULTS：At different zymolytic times,most of their mycelia grew normally and quickly,especially number A,C,D,E,F,M,Q,V,SW,い and so on.The contents of aconitine,mesaconine and hypaconitine in fermented A.carmichaeli were significantly lower than in A.carmichaeli.CONCLUSION：The contents of aconitine,mesaconine and hypaconitine in ＂medicinal mycoplasma＂ have been decreased significantly.Two-way fermentation is conducive exploration of biotechnology in the filed of traditional Chinese medicine.