| HPLC法同时测定麦冬药材中麦冬皂苷Ra和慈溪麦冬皂苷A的含量 |
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| 引用本文： | 胥秀英,郑一敏,傅善权,韩玉梅,杨艳红. HPLC法同时测定麦冬药材中麦冬皂苷Ra和慈溪麦冬皂苷A的含量[J]. 中国药房, 2010, 0(39): 3704-3705 |
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| 作者姓名： | 胥秀英 郑一敏 傅善权 韩玉梅 杨艳红 |
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| 作者单位： | 重庆理工大学药学与生物工程学院,重庆市400050 |
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| 基金项目： | 重庆市科技攻关计划项目（CSTC2008AC5029 CSTC2008AB5116） |
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| 摘 要： | 目的:建立以高效液相色谱法测定麦冬药材中麦冬皂苷Ra和慈溪麦冬皂苷A含量的方法。方法:色谱柱为Waters C18(150mm×4.6mm,5μm),流动相为乙腈-水(35∶65),流速为1.0mL·min-1,检测波长为208nm,柱温为室温。结果:麦冬皂苷Ra、慈溪麦冬皂苷A的进样量分别在1.88～9.40μg(r=0.9996)、1.96～9.80μg(r=0.9995)范围内与各自峰面积积分值呈良好线性关系;平均回收率分别为100.2%、99.7%,RSD均为1.7%(n=6)。结论:本方法简便、快速、准确,可用于麦冬药材的质量控制。
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| 关 键 词： | 麦冬 高效液相色谱法 麦冬皂苷Ra 慈溪麦冬皂苷A 含量测定 |
Content Determination of Ophiopogon Ra and Cixi-ophiopogon A from Ophiopogon japonicus by HPLC |
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| XU Xiu-ying,ZHENG Yi-min,FU Shan-quan,HAN Yu-mei,YANG Yan-hong. Content Determination of Ophiopogon Ra and Cixi-ophiopogon A from Ophiopogon japonicus by HPLC[J]. China Pharmacy, 2010, 0(39): 3704-3705 |
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| Authors: | XU Xiu-ying ZHENG Yi-min FU Shan-quan HAN Yu-mei YANG Yan-hong |
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| Affiliation: | (College of Pharmaceutical and Biological Engineering,Chongqing University of Technology,Chongqing 400050,China) |
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| Abstract: | OBJECTIVE：To determine the contents of ophiopogon Ra and cixi-ophiopogon A from Ophiopogon japonicus by HPLC.METHODS：The determination was performed on Waters C18（150 mm×4.6 mm,5μm） column with mobile phase consisted of acetonitrile-water（35：65） at flow rate of 1.0 mL·min^-1.The detection wavelength was set at 208 nm and the column temperature was room temperature.RESULTS：The linear range of ophiopogon Ra was 1.88～9.40 μg（r=0.999 6） with an average recovery of 100.2%（RSD=1.7%,n=6）.The liner range of cixi-ophiopogon A was 1.96～9.80 μg（r=0.999 5） with an average recovery of 99.7%（RSD=1.7%,n=6）.CONCLUSION：The method is simple,rapid and accurate for the quality control of O.japonicus. |
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| Keywords: | Ophiopogon japonicus HPLC Ophiopogon Ra Cixi-ophiopogon A Content determination |
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