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不同品种附子生物碱和多糖含量的比较
引用本文:舒晓燕,侯大斌,李凤. 不同品种附子生物碱和多糖含量的比较[J]. 中国药房, 2010, 0(31): 2916-2918
作者姓名:舒晓燕  侯大斌  李凤
作者单位:西南科技大学生命科学与工程学院,绵阳市621000
基金项目:国家支撑计划课题(2007BAD89B15); 四川省农作物育种攻关课题(2006YZGG12-22)
摘    要:
目的:比较不同品种附子生物碱和多糖的含量。方法:采用酸性染料比色法测定附子总生物碱含量,反相高效液相色谱法测定双酯型生物碱含量,蒽酮-硫酸法测定多糖含量。结果:不同品种附子总生物碱和双酯型生物碱含量差异较大,附子多糖含量差异不显著。附子炮制后,黑附片中总生物碱、双酯型生物碱含量显著下降,附子多糖含量稍有增加。结论:本试验结果可为附子品种选育提供一定的科学依据。

关 键 词:附子  品种  总生物碱  双酯型生物碱  多糖  含量

Study on the Content of Alkaloids and Polysaccharide in Different Varieties of Aconitum carmichaeli
SHU Xiao-yan,HOU Da-bin,LI Feng. Study on the Content of Alkaloids and Polysaccharide in Different Varieties of Aconitum carmichaeli[J]. China Pharmacy, 2010, 0(31): 2916-2918
Authors:SHU Xiao-yan  HOU Da-bin  LI Feng
Affiliation:(College of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621000, China)
Abstract:
OBJECTIVE: To research the content of alkaloids and polysaccharides in different varieties of Aconitum carmichaeli. METHODS: The contents of total alkaloids, diester diterpenoid alkaloids and polysaccharides were measured by acid dye colorimetry, HPLC and sulphuric acid-anthrone colorimetric method respectively. RESULTS: In different varieties, the differences of total alkaloids and diester diterpenoid alkaloids were significant. However, the difference of polysaccharides wasn’t significant. The content of total alkaloids and diester diterpenoid alkaloids in processed A. carmichaeli reduced significantly while the content of polysaccharides increased slightly. CONCLUSION: The results of study could provide a scientific basis for rational breeding of A. carmichaeli.
Keywords:Aconitum carmichaeli  Varieties  Total alkaloids  Diester diterpenoid alkaloids  Polysaccharide  Content
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