HPLC法测定奥沙利铂脂质体中奥沙利铂的含量

目的:建立以高效液相色谱法测定奥沙利铂脂质体中奥沙利铂含量的方法。方法:色谱柱为Hypersil C18(250 mm×4.6mm,5μm),流动相为甲醇:水=5∶95(V/V),流速为1.0 mL·min-1,检测波长为250 nm,柱温为30℃。结果:奥沙利铂的检测浓度在12.2～122.0μg·mL-1范围内线性关系良好;以峰面积(Y)为对浓度(C)进行回归分析,得回归方程为Y=78 575 C-4 811.8(n=5,r=0.999 9),奥沙利铂的检测浓度在12.2～122.0μg·mL-1范围内线性关系良好;检测限为97.6 ng·mL-1;平均回收率为98.58%,RSD=1.83%。结论:该方法操作简便、灵敏、准确,可用于奥沙利铂脂质体中奥沙利铂的含量测定。

Content Determination of Oxaliplatin in Oxaliplatin Liposome by HPLC

OBJECTIVE：To establish an HPLC method for the content determination of oxaliplatin in Oxaliplatin liposome. METHODS：The separation was performed on Hypersil C18（250 mm×4.6 mm,5 μm） column with methanol-water（5 ∶ 95,V/V） as mobile phase at flow rate of 1.0 mL·min^-1. The detection wavelength was set at 250 nm and column temperature was 30 ℃. RESULTS：The linear range of oxaliplatin was 12.2～122.0 μg.mL^-1（n=5,r=0.999 9） with an average recovery of 98.58%（RSD=1.83%）. The limit of detection（LOD） was 97.6 ng·mL^-1. CONCLUSION：The method is simple,sensitive and accurate for the content determination of oxaliplatin in Oxaliplatin liposome.