| HPLC法测定不同产地叶下珠中槲皮素的含量 |
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| 引用本文： | 王琳,朱缨,范淦彬. HPLC法测定不同产地叶下珠中槲皮素的含量[J]. 中国药房, 2010, 0(39): 3717-3718 |
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| 作者姓名： | 王琳 朱缨 范淦彬 |
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| 作者单位： | [1]苏州卫生职业技术学院,苏州市215009 [2]苏州派腾生物医药科技有限公司,苏州市215009 |
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| 基金项目： | 苏州卫生职业技术学院科技项目课题（Szwzy200713） |
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| 摘 要： | 目的:建立以高效液相色谱(HPLC)法测定不同产地叶下珠中槲皮素含量的方法。方法:色谱柱为ZorbaxSB-C18(250mm×4.6mm,5.0μm),流动相为乙腈-0.2%磷酸(30:70),流速为1mL·min-1,紫外检测波长为370nm。结果:槲皮素进样量在0.05～0.40μg范围内与峰面积积分值呈良好的线性关系(r=0.9999);平均回收率为102.23%,RSD=1.87%(n=6)。云南产的叶下珠中槲皮素的含量最高。结论:本方法简便、快速、准确,可为正确选择叶下珠原料及其综合开发提供科学依据。
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| 关 键 词： | 叶下珠 槲皮素 高效液相色谱法 含量测定 产地 |
Content Determination of Quercetin in Phyllanthus urinaria from Different Habitats by HPLC |
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| WANG Lin,ZHU YingSuzhou Heath College,Suzhou,China FAN Gan-bin. Content Determination of Quercetin in Phyllanthus urinaria from Different Habitats by HPLC[J]. China Pharmacy, 2010, 0(39): 3717-3718 |
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| Authors: | WANG Lin ZHU YingSuzhou Heath College Suzhou China FAN Gan-bin |
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| Affiliation: | WANG Lin,ZHU Ying(Suzhou Heath College,Suzhou 215009,China) FAN Gan-bin(Suzhou Patent Biological and Pharmaceutical Technology Co.,Ltd.,Suzhou 215009,China) |
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| Abstract: | OBJECTIVE：To develop a HPLC method for content determine of quercetin in Phyllanthus urinaria from different habitats.METHODS：The determination was performed on Zorbax SB-C18（250 mm×4.6 mm,5.0 μm） column with acetonitrile-0.2% phosphoric acid（30：70） as mobile phase at flow rate of 1 mL·min-1.The detection wavelength was set at 370 nm.RESULTS：The linear range of quercetin was 0.05～0.40 μg（r=0.999 9） with an average recovery of 102.23%（RSD=1.87%,n=6）.CONCLUSION：The method is simple,rapid and accurate.It provides scientific basis for the selection of raw material and comprehensive development of P.urinaria. |
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| Keywords: | Phyllanthus urinaria Quercetin HPLC Content determination Habitats |
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