消瘤1号诱导MCF-7人乳腺癌细胞凋亡的研究

目的:研究中成药消瘤1号诱导MCF-7人乳腺癌细胞的凋亡作用,并通过分析其作用后MCF-7细胞Bax和Bcl-2蛋白表达的变化,探讨其诱导MCF-7细胞凋亡的分子机制。方法:采用细胞培养技术,用1、10、100、1000μg·mL-1的消瘤1号处理MCF-7细胞24h,PI染细胞,流式细胞仪测定细胞周期的变化,Western Blot法检测Bax和Bcl-2蛋白的表达。结果:流式细胞仪检测结果表明细胞阻滞在G0/G1期;Weston Blot法测定结果表明消瘤1号能够增加细胞中Bax蛋白表达,减少Bcl-2蛋白表达,且该2种蛋白的表达依赖于消瘤1号的浓度。结论:消瘤1号可通过调节Bax/Bcl-2蛋白表达的变化诱导MCF-7细胞凋亡。

Study on Effect of Xiaoliu Formula I on the Apoptosis of MCF-7 Human Breast Cancer Cells

OBJECTIVE： To investigate the effects of Xiaoliu formula I on the apoptosis of MCF-7 human breast cancer cell, and to explore the molecular mechanism of the apoptosis of MCF-7 human breast cancer cell by analyzing the protein expression of Bax and Bcl-2 in MCF-7. METHODS： MCF-7 cells were treated with compounds of 1 μg·mL^-1,10 μg·mL^-1, 100μg·mL^-1, 1 000 μg·mL^-1, and PI staining was conducted. Cell cycle was assayed by FACS, and Bax and Bcl-2 protein expression were analyzed by Western blot. RESULTS： The results of FACS showed that cells were retarded in G0/G1 phase after treating with Xiaoliu formula I for 24 h. Results of Western blot revealed that Xiaoliu formula I improved the protein expression of Bax and reduced protein expression of Bcl-2 with concentration-dependent manner. CoNcLusION ： Xiaoliu formula I can induce the apoptosis of MCF-7 by regulating the protein expression of Bax/Bcl-2.