5-FU增强TRAIL诱导的胃癌BGC823细胞凋亡的实验研究

目的 观察5-FU对TRAIL诱导的胃癌BGC823细胞凋亡的影响,明确死亡受体5(DR5)在5-FU和TRAIL诱导凋亡中的作用.方法 采用MTT法测定细胞活力、流式细胞仪检测细胞凋亡、免疫印迹检测蛋白表达.结果 TRAIL可导致BGC823细胞轻度的增殖抑制和少量的细胞凋亡.与单药TRAIL和5-FU相比,TRAIL联合5-FU对细胞的增殖抑制和诱导凋亡作用明显增强(P＜0.05).免疫印迹结果显示,TRAIL没有改变DR5的蛋白表达,而5-FU作用BGC823细胞48 h后,DR5蛋白表达上调(P＜0.05).TRAIL和5-FU联合作用后,DR5蛋白表达同样明显上调(P均＜0.05).结论 5-FU通过上调DR5蛋白表达提高了BGC823细胞对TRAIL的敏感性.

TRAIL-induced apoptosis enhanced by 5-FU in gastric cancer BGC823 cells

Objective To observe the inhibition effect of 5-FU on tumor necrosis factor-related apoptosis-inducing ligand(TRAIL)-induced apoptosis in gastric cancer BGC823 cells,and identify the action of death receptor 5(DR5) in 5-FU and TRAIL-induced apoptosis.Methods Cell proliferation was measured using MTT assay.Cell apoptosis was determined by flow cytometry.Expression of protein was analyzed by western blot.Results Treatment with 100 ng/mL TRAIL for 48 h slightly resulted in the inhibition of cell proliferation and a little cell apoptosis in BGC823 cells.Treatment with TRAIL(100 ng/mL) and 5-FU(5.55 g/mL,IC50 dose of 48 h) leaded to a dramatic increase in the inhibitiong of cell proliferation and cell apoptosis compared to treatment with 5-FU or TRAIL alone(P<0.05).TRAIL alone did not change the expression of DR5,while 5.55 g/mL 5-FU significantly upregulated the expressions of DR5 in BGC823 cells after 48 h(P<0.05).Treatment with 5-FU and TRAIL also resulted in the upregulation of DR5(all P<0.05).Conclusion 5-FU enhance TRAIL-induced apoptosis in gastric cancer BGC823 cells by the upregulation of DR5.