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Effect of topical application of fluoride gel NaF 2% on enzymatic and non-enzymatic antioxidant parameters of saliva
Authors:Leite Mariana Ferreira  Ferreira Nayara Ferraz D'Assumpção  Shitsuka Caleb David Willy Moreira  Lima Amanda Martins  Masuyama Mônica Miyuki  Sant'Anna Giselle Rodrigues  Yamaguti Paula Mochidome  Polotow Tatiana G  de Barros Marcelo Paes
Affiliation:1. Horticultural Department, Nanjing Agricultural University, Nanjing 210095, China;2. State Key Laboratory of Crop Genetics & Germplasm Enhancement, Nanjing 210095, China;3. Key Laboratory of Southern Vegetable Crop Genetic Improvement, Ministry of Agriculture, Nanjing 210095, China;4. Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210014, China;1. Departamento de Bioquímica e Biologia Tecidual, UNICAMP, Campinas, SP, Brazil;2. Laboratório de Bioquímica e Química de Proteínas, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, DF, Brazil;3. Centro Nacional de Pesquisa em Energia e Materiais, Laboratório Nacional de Biociências, Campinas, SP, Brazil
Abstract:
ObjectiveThe aim of the study was to evaluate the effect of topical fluoride gel NaF 2% application on antioxidant parameters of whole saliva from children.DesignThe saliva mechanically stimulated with parafilm was collected from 25 children (6–12 years) attending the Clinic of Paediatric Dentistry of Universidade Cruzeiro do Sul, São Paulo, Brazil, before (control group) and immediately after application of neutral fluoride gel NaF 2% (fluoride-gel group), according to the Standards for Research Using Human Subjects, Resolution 196/96 of the USA National Health Council of 10/10/1996. Afterwards, pre-post ferric-reducing antioxidant power (FRAP), trolox-equivalent antioxidant capacity (TEAC), uric acid, reduced/oxidised glutathione content (GSH/GSSG) and total peroxidase activity (TPO) were evaluated in whole saliva of both groups.ResultsAll non-enzymatic antioxidant parameters were augmented by fluoride-gel NaF 2% application, whereas a notable reduction (31%) of peroxidase activity was concomitantly observed in the children's saliva (p  0.05). Nevertheless, the reducing power of saliva was kept unaltered under these circumstances (p  0.05).ConclusionsDespite the reduced activity of peroxidase (an important antimicrobial and antioxidant enzyme), the topical fluoride gel NaF 2% favourably stimulated the release of non-enzymatic antioxidant components of saliva, sustaining the reducing power of saliva and the natural defences of the oral cavity.
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