成人骨髓间充质干细胞体外多向分化潜能特性的研究

目的研究体外不同诱导条件下成人骨髓间充质干细胞向成骨细胞、脂肪细胞、神经细胞分化的能力。方法用细胞化学方法对诱导后的骨髓细胞进行脂肪细胞特异油红O染色；Von Kossa及改良的钙钴法进行成骨细胞鉴定；免疫组化方法检测诱导后的骨髓细胞神经元特异性烯醇化酶(NSE)、神经丝蛋白-M(NF—M)、胶质纤维酸性蛋白(GFAP)表达。结果在不同的诱导条件下，诱导后的细胞油红O染色胞浆内可见橙红色脂滴；Von Kossa染色可见细胞间布满黑色颗粒，提示有矿化基质沉积；改良钙钻法碱性磷酸酶染色胞浆呈深棕色或深黑色。免疫组化染色显示向神经细胞方向诱导后细胞NSE( )、NF—M( )、GFAP(-)。结论成人骨髓间充质干细胞在体外具有多向分化的潜能。

Study on multipotential differentiation of human bone marrow mesenchymal stem cells in vitro

Objective To study the capability of human bone marrow mesenchymal stem cells(MSCs) to differentiate into adipocyte, osteoblasts, and neurocytes under different experimental conditions in vitro. Methods The induced human bone marrow MSCs were examined by cytochemistry staining and immunohistochemistry staining. Results Induced human bone marrow MSCs formed alkaline phosphatasepositive aggregates and Von Kossa stainpositive nodules under the condition of osteogenic differentiation. Under the condition of adipogenic differentiation,the isolated human bone marrow MSCs formed oil redOpositive cells. Immunohistochemistry results showed that differentiated human bone marrow MSCs expressed neuronspecific enolase (NSE) and neurofilament (NFM) . Conclusion Human bone marrow MSCs have the capability of multipotential differentiation in vitro.