急性酒精性肝损伤与肝细胞凋亡的研究

目的研究急性酒精性肝损伤肝细胞凋亡,探讨其发病机制。方法将30只大鼠随机分成酒精组(20只),对照组(10只)。给酒精组大鼠按体重15 g/kg 47.5%酒精一次性灌胃,给对照组大鼠按体重15 g/kg生理盐水一次性灌胃。灌胃后48 h处死大鼠,用光镜、TUNEL、电镜检查肝组织。结果酒精组肝损伤、肝细胞凋亡检出率均为100%,凋亡肝细胞核数占肝细胞核总数的46.8%。生理盐水组未发现肝损伤病变,仅3例个别视野近肝小叶中央静脉处见单个凋亡细胞。结论肝细胞凋亡与急性酒精性肝损伤有一定的关系。

Study on hepatocyte apoptosis in acute alcoholic liver injury

OBJECTIVE To explore the pathogenesis of hepatocyte apoptosis in acute alcoholic liver injury(AALI).METHODS 30 rats were randomly divided into the alcohol group of 20 rats and the control group of 10 rats.The alcohol group was given the alcohol(47.5% v/v)at the dose of 15g/kg body weight by gavages,and the control group was given the physiological saline.These rats were killed after 48h.Their hepatic tissues were examined by light microscope,TUNEL and electronic microscope.RESULTS The liver injury and hepatocyte apoptosis were found in all rats in the alcohol group,and the rate of hepatocyte nuclei with positive apoptosis was 46.8% in the total hepatocyte nuclei.The liver injury was not found in all rats of the control group while a few hepatocyte apoptosis in the 3 rats was found near the central veins of the hepatic lobule.CONCLUSION The hepatocyte apoptosis is an important cause for acute alcoholic liver injury.