| 胸腺内注射Fas抗体对心脏移植大鼠Fas/FasL表达的影响 |
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| 引用本文： | 王秋艳,满迎春,张国春,张晶敏. 胸腺内注射Fas抗体对心脏移植大鼠Fas/FasL表达的影响[J]. 华南国防医学杂志, 2009, 23(6): 21-24 |
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| 作者姓名： | 王秋艳 满迎春 张国春 张晶敏 |
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| 作者单位： | 秦皇岛港口医院心内科,河北秦皇岛,066002;黑龙江省第二肿瘤医院肿瘤内科;哈尔滨市第二人民医院心内科;黑龙江省海伦市人民医院心内科 |
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| 摘 要： | 目的研究受体胸腺内注射Fas抗体对心脏移植大鼠Fas/FasL表达的影响。方法应用大鼠同种异位腹腔心脏移植模型,将24只模型动物随机均分为三组,A组移植前后不给处置,B组给环孢素A,按每日8mg/kg的剂量于术前一天开始口服,C组胸腺注射Fas抗体。观察术后移植心脏局部的Fas及FasL的表达情况（免疫组化法和RT-PCR法）。结果与A组相比,C组移植心脏局部的Fas/FasL基因表达（RT-PCR法半定量结果P＜0.05）和蛋白表达减少（免疫组化法P＜0.05）。结论受体胸腺内注射Fas抗体是一种有效的抑制心脏移植急性排斥反应的方法,引起移植心肌局部Fas/FasL表达下调。
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| 关 键 词： | Fas抗体 心脏移植 急性排斥反应 Fas/FasL |
Influence of Intrathymic Injection of Anti-Fas Monoclonal Antibody on Fas/FasL in Rats after Heart Transplantation |
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| Affiliation: | WANG Qiu-yan , MAN Ying-chun, ZHANGGuo chun, et al. (1 Department of Cardiology, Qinhuangdao Port Hosipital of Hebei Province, Qinghuangdao Hebei 066002, China) |
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| Abstract: | Objective To investigate the influence of intrathymic injection of anti-Fas monoclonal antibody on Fas/FasL in rats after heart transplantation. Methods Allograft heterotopic heart transplantation models of rats were utilized. Twenty four rats were randomly and evenly divided into three groups. Group A had no special perioperation inter vention. Group B was administrated with cyclosporine a 8 mg/（kg × d） from the day before operation. Group C was injected with Fas antibody in thymus. The expression of Fas/FasL on the local tissue of transplanted heart after operations was observed. Results Compared to group A, group C had less gene expression （RT-PCR, semiquantitative, P〈0. 05） and less protein expression （immunohistochemistry, P〈0. 05） of Fas/FasL on the local transplanted heart. Conclusion Intrathymic injection of anti Fas monoclonal antibody has a immunosuppressive effect on acute rejection reaction and down-regulation on the expression of Fas/FasL in rats after heart transplantation. |
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| Keywords: | Fas/FasL |
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