Detection of rabies-specific antigens by egg yolk antibody (IgY) to the recombinant rabies virus proteins produced in Escherichia coli |
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| Authors: | Motoi Yurie Inoue Satoshi Hatta Hajime Sato Kozue Morimoto Kinjiro Yamada Akio |
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| Affiliation: | The United Graduate School of Veterinary Science, Gifu University, Gifu 501-1193, Japan. |
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| Abstract: | We obtained rabies-specific egg yolk antibodies (IgY) by immunizing hens with recombinant His-tagged nucleoprotein and phosphoprotein (rN, rP) of the rabies virus (CVS-11 strain) expressed in Escherichia coli. The anti-rN and rP IgY were shown to bind specifically to the respective proteins of the CVS-11 strain of rabies virus by Western blotting, immune fluorescent assay and immunohistochemistry, indicating that IgY to rabies recombinant proteins could serve as a reagent for diagnosis of rabies virus infection. |
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