铜绿假单胞菌临床分离株药敏试验结果分析

目的分析2010~2011年临床标本中分离出的铜绿假单胞菌药敏试验结果,为临床预防感染以及合理用药提供指导依据.方法严格按照《全国临床检验操作规程》和德灵细菌鉴定及药敏分析仪操作说明对送检的临床标本进行细菌学培养鉴定与药敏试验分析.结果两年间从各类临床标本中分离出247株铜绿假单胞菌,分离率为9.56%,主要来源于呼吸道标本(71.26%).铜绿假单胞菌对氨苄西林、氨苄西林/舒巴坦、头孢呋辛、头孢替坦、头孢唑啉、复方新诺明呈高耐药性,耐药率在90%以上;而哌拉西林/他唑巴坦、美洛培南及亚胺培南对铜绿假单胞菌耐药率较低,分别为21.46%、21.46%、20.24%.此外,头孢他啶、环丙沙星、妥布霉素等耐药率在30%~40%.结论铜绿假单胞菌呈多重耐药性,根据药敏结果合理使用抗菌药物具有重要临床指导意义.

Analysis of the drug susceptibility test on clinical isolated Pseudomonas aeruginosa

Objective To analyze the susceptibility test results of Pseudomonas aeruginosa isolated from clinical specimens from 2010 to 2011 years,which provide guidance for doctors.Methods All collected clinical specimens were strictly done for bacteriological culture and identification and susceptibility test analysis,according to the operating instructions of the clinical laboratory operating procedures,VITEK2 automatic bacterial identification and susceptibility analyzers operating instructions.Results 247 Pseudomonas aeruginosa were isolated from a variety of clinical specimens in recent 2 years,which account for 9.56% in all specimens.And they were mainly seperated from respiratory specimens(71.26%).In the study,Pseudomonas aeruginosa showed a high resistance to some antimicrobial agents,including ampicillin,cefuroxime,cefotetan,cefazolin,cotrimoxazole and so on,which were more than 90% in the resistance ratios.However,piperacillin/tazobactam,meropenem and imipenem showed about 20% resistance ratios for Pseudomonas aeruginosa.In addition,the resistance ratios of ceftazidime,ciprofloxacin,tobramycin and other drug were around 30%-40%.Conclusion Pseudomonas aeruginosa has multiple drug resistance.This study has important clinical significance for the rational use of antibiotics.