实时荧光定量聚合酶链反应在肺结核诊断中的应用

目的探讨实时荧光定量聚合酶链反应(FQ-PCR)在肺结核诊断中的应用价值。方法应用FQ-PCR对临床诊断肺结核及其他肺部疾病患者的临床标本进行结核分枝杆菌DNA定量检测,同时与涂片抗酸染色、BACTEC 960培养法作比对。结果 189例临床诊断肺结核患者FQ-PCR检测123例阳性,提示敏感度为65.1%(123/189),而涂片抗酸染色、BACTEC 960培养法分别为40.2%(76/189)、63.5%(120/189)。94例其他肺部疾病患者及20例健康对照者FQ-PCR检测均为阴性,特异度为100.0%。结论 FQ-PCR敏感度和特异度均高,检测过程简单、易标准化、全过程仅需4～5h,可用于结核病的快速诊断。

Application of Real-time fluorescence quantitative PCR in diagnosis of mycobacterium tuberculosis

Objective The aim of study was to evaluate the clinical value of diagnosis of mycobacterium tuber- culosis by real-time fluorescence quantitative PCR. Methods Real time fluorescence quantitative PCR was used to detect the copies of TB DNA of the specimens from M. tuberculosis patients and other patients with non-tuberculosis respiratory system disease,and it was also compared with the two methods of smear acid-fast stain and culture with BACTEC MGIT 960 system. Results Of all the 189 M. tuberculosis patients, 123 cases were positive by real-time fluorescence quantitative PCR, the sensitivity was 65.1% （123/189）. The sensitivities of smear acid-fast stain, culture with BACTEC MGIT 960 system were 40.2~//oo （76/189）, 63.5 % （120/189）. 94 cases of other pulmonary diseases and 20 cases of healthy subjects were negative, the specificity of the method was 100%. Conclusion Real-time fluores- cence quantitative PCR showed high specificity and sensitivity for diagnosis tuberculosis. The process is simple and easy to standardize. The whole process only demanded 4-5 hours. It is very suitable for rapidly diagnosis of tuberculo- sis.