An enzyme-linked immunosorbent assay for the quantitation of human IgG subclasses using monoclonal antibodies |
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| Authors: | A Ferrante B Rowan-Kelly L J Beard G M Maxwell |
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| Affiliation: | 1. Department of Paediatrics, University of Adelaide, Adelaide Children''s Hospital, Adelaide, S.A. 5006, Australia;2. Department of Immunology, Adelaide Children''s Hospital, Adelaide, S.A. 5006, Australia;1. Environmental and Chemical Engineering, Faculty of Technology, University of Oulu. Oulu, Finland;2. Nano and Molecular Systems Research Unit (NANOMO), Faculty of Science, University of Oulu. Oulu, Finland;3. NanoTech – Nanomaterials and Sustainable Chemical Technologies. Department of Inorganic Chemistry, Faculty of Science, University of Granada, Avda. Fuente Nueva, 18071, Granada, Spain;1. Groupe de recherche en écologie buccale (GREB), faculté de médecine dentaire, université Laval, 2420, rue de la Terrasse, G1V 0A6 Quebec, Canada;2. Centre de recherche en infectiologie porcine et avicole (CRIPA), fonds de recherche du Québec – nature et technologie (FRQNT), J2S 2M2 Saint-Hyacinthe, Quebec, Canada;1. Institute for Bioscience and Biotechnology Research, University of Maryland, Rockville, MD 20850, USA;2. Department of Veterinary Medicine, University of Maryland, College Park, MD 20742, USA;1. Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam 781039, India;2. Center for Medical Biotechnology, M.D. University, Rohtak 124001, Haryana, India;3. College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary & Animal Sciences, Hisar 125004, Haryana, India |
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| Abstract: | An enzyme-linked immunosorbent assay was established for the quantitation of human IgG1, IgG2, IgG3 and IgG4 using IgG subclass-specific monoclonal antibodies. The method could detect 1-10 ng/ml of the Ig subclasses. The technique is suitable for measuring IgG subclass concentration in sera of healthy adults and in supernatants from human lymphocytes cultured in the presence of pokeweed mitogen. |
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