Molecular cloning and complete nucleotide sequence of the genome of Japanese encephalitis virus Beijing-1 strain |
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| Authors: | Hiroshi Hashimoto Akio Nomoto Koji Watanabe Takayuki Mori Toshiyuki Takezawa Chikara Aizawa Tsutomu Takegami Keiichi Hiramatsu |
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| Affiliation: | (1) Department of Virology, The Kitasato Institute, Shirokane 5-9-1, Minato-ku, 108 Tokyo, Japan;(2) Department of Microbiology, Tokyo Metropolitan Institute of Medical Science, Honkomagome, Bunkyo-ku, 113 Tokyo, Japan;(3) Kanazawa Medical University Uchinada-cho, Kahoku-gun, 920 Ishikawa, Japan;(4) Tokyo Medical and Dental University, Yushima, Bunkyo-ku, Tokyo, Japan |
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| Abstract: | The genomic RNA of the Japanese encephalitis virus (JEV) Beijing-1 strain was reversely transcribed and the synthesized cDNA was molecularly cloned. Six continuous cDNA clones that cover the entire virus genome were established and sequenced to determine the complete nucleotide sequence of the JEV RNA. The precise genomic size was estimated as 10,965 bases long. With flanking 95 bases at the 5 and 583 bases at the 3 non-coding regions, one long open reading frame (ORF) was revealed encoding a virus polyprotein with 3,429 amino acid residues. Because of sequence homologies observed between JEV and other flaviviruses, the genome organization of JEV appears to be identical with other flaviviruses. Genetic variation detected among flavivirus genomes is consistent with the established serological relatedness between JEV and other members of flaviviruses. The secondary structure of the JEV genome is deduced and discussed concerning its involvement in genome replication. |
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| Keywords: | JEV genome molecular cloning, genome sequence translation strategy genome organization secondary structure |
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