吸烟对人体外周血白介素2及其受体系统影响研究

选取北京城区及近郊４２例健康吸烟者和与之年龄、生活方式相配对的健康非吸烟者作为研究对象，分离其外周血单核细胞（ＰＢＭＣＳ），在３７℃、５％ＣＯ２条件下经植物血凝素（ＰＨＡ）刺激活化。采用免疫化学技术检测活化４８小时后的白介素２（ＩＬ－２）分泌细胞百分数、活化７２小时后的膜白介素２受体（ｍＩＬ－２Ｒ）阳性细胞百分数和血清中可溶性白介素２受体（ｓＩＬ－２Ｒ）水平。结果显示：在体外条件下，吸烟组ｍＩＬ－２Ｒ阳性细胞百分数显著高于非吸烟组（Ｐ＜０．０５）；血清中ｓＩＬ－２Ｒ水平吸烟组显著高于非吸烟组（Ｐ＜０．０５）；吸烟组ＩＬ－２分泌细胞百分数有高于非吸烟组的趋势（Ｐ＝０．０８）。指标的变化与吸烟的状况也有一定的关系。提示吸烟引起吸烟者体内作为细胞因子网络重要环节之一的ＩＬ－２及其受体系统发生改变

Effects of Smoking on Interleukin 2 and Its Receptor System in Human Peripheral Blood

42 healthy male smokers and 42 healthy male nonsmokers matched in age and life style were strictly chosen into the study from Beijing urban and rural areas. Peripheral blood mononuclear cells(PBMCs) were separated and then stimulated by PHA under 37 degrees C, 5% CO2 condition. Immunochemical methods were applied in testing the synthesis of interleukin 2(IL-2) and the expression of its membrane receptor (mIL-2R) and the level of soluble receptor (sIL-2R) in serum. The results showed that when compared with nonsmokers, stimulated PBMC from smokers had significant higher percentage of mIL-2R positive cells (P < 0.05) in vitro. Levels of sIL-2R in serum of smokers were also significantly higher than those of nonsmokers (P < 0.05). The percentage of IL-2 secrete cells of smokers had higher tendency (P = 0.08). These data indicated that the immune function of smokers and nonsmokers are different concerning the IL-2 and its receptor system.