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| 应用显微荧光测钙技术研究乙酰胆碱对豚鼠耳蜗对外毛细胞内 … | |
| 引用本文： | 杨军 汪吉宝. 应用显微荧光测钙技术研究乙酰胆碱对豚鼠耳蜗对外毛细胞内 …[J]. 临床耳鼻咽喉头颈外科杂志, 1998, 12(6): 271-273 |
| 作者姓名： | 杨军 汪吉宝 |
| 摘 要： | 介绍了应用最显微荧光测钙技术研究豚鼠耳蜗外毛细胞（ＯＨＣ）内游离钙浓度的变化的方法。该技术以钙敏感染料Ｆｕｒａ－２作为胞内Ｃａ＾２＋的指示剂，装协进入细胞后，与胞内游离Ｃａ＾２＋结合，在特定波长光的激发下可发出荧光，由此可推算「Ｃａ＾２＋」ｉ的变化。结果发现乙酰胆碱在含钙的细胞上液中可引起「Ｃａ＾２＋」ｉ升高。对显微荧光测钙技术在研究耳蜗毛细胞中的应用以及ＡＣｈ引起「Ｃａ＾２＋」ｉ升高的机制进行讨 |
| 关 键 词： | 显微荧光 钙 耳蜗外毛细胞 乙酰胆碱 |
Study on changing of intracellular free Ca2+ induced by ACh in outer hair cells of guinea pig using Fura-2 microfluorimetry] |
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| J Yang,J Wang. Study on changing of intracellular free Ca2+ induced by ACh in outer hair cells of guinea pig using Fura-2 microfluorimetry][J]. Journal of clinical otorhinolaryngology, head, and neck surgery, 1998, 12(6): 271-273 | |
| Authors: | J Yang J Wang |
| Affiliation: | Department of Otorhinolaryngology, Union Hospital, Tongji Medical University, Wuhan 430022. |
| Abstract: | The method of studying intracellular free Ca2+ ([Ca2+]i) in outer hair cells (OHC) of guinea pig using digital imaging microscopy and Ca(2+)-sensitive fluorescent dye Fura-2 was introduced. [Ca2+]i can be calculated from fluorescence intensity ratios with excitation lights of two different wave-lengths, using Fura-2 loaded in OHC. The data showed that acetylcholine (ACh) can induce [Ca2+]i elevation of OHC in the solution with calcium. Mechanism of ACh inducing [Ca2+]i elevation and application of calcium imaging microfluorometry for studying cochlea hair cell were discussed. |
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