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辛伐他汀对大鼠急性心肌梗死后心肌白细胞介素-6表达的影响
引用本文:张金盈,廖玉华,程翔,逯保军. 辛伐他汀对大鼠急性心肌梗死后心肌白细胞介素-6表达的影响[J]. 郑州大学学报(医学版), 2005, 40(1): 25-28
作者姓名:张金盈  廖玉华  程翔  逯保军
作者单位:1. 华中科技大学同济医学院心血管病研究所,协和医院心内科,武汉,430022;郑州大学第一附属医院心内科,郑州,450052
2. 华中科技大学同济医学院心血管病研究所,协和医院心内科,武汉,430022
3. 郑州大学第二附属医院心内科,郑州,450003
基金项目:国家自然科学基金资助项目 30370574,河南省医学科技创新人才工程项目 2002116,郑州大学科研发展基金资助项目 2004021
摘    要:目的:观察辛伐他汀对大鼠急性心肌梗死(AMI)后心肌组织白细胞介素- 6 (IL -6)基因及其蛋白表达的影响。方法:Wistar大鼠随机分 3组:辛伐他汀治疗组(MI- S组)、梗死对照组(MI C组)和假手术组 (Sham组 )。前2组大鼠结扎左冠状动脉前降支(LAD)制成AMI模型,MI S组给辛伐他汀 40mg/kg灌胃治疗,余 2组给予生理盐水灌胃。4周后,RT- PCR法和免疫组织化学法分别检测左心室梗死及非梗死区IL- 6mRNA和蛋白的表达,Westernblot法测定左心室非梗死区心肌IL- 6蛋白表达。结果:大鼠AMI后 4周,MI C和MI S组左心室梗死及非梗死区心肌IL- 6mRNA及蛋白表达均较Sham组明显升高(P<0. 05),MI S组IL- 6mRNA及蛋白表达均较MI C组低 (P<0. 05)。3组血脂差异无统计学意义。结论:辛伐他汀可减少AMI大鼠左心室心肌IL 6mRNA及蛋白表达,此改变不依赖其降脂作用。

关 键 词:辛伐他汀 心肌梗死 白细胞介素-6 RT-PCR 免疫组化 大鼠
修稿时间:2004-09-22

Influence of simvastatin on interleukin-6 mRNA expression in rats after acute myocardial infarction
ZHANG Jinying ,),LIAO Yuhua ),CHENG Xiang ),LU Baojun ) )Institute of Cardiology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan ). Influence of simvastatin on interleukin-6 mRNA expression in rats after acute myocardial infarction[J]. Journal of Zhengzhou University: Med Sci, 2005, 40(1): 25-28
Authors:ZHANG Jinying   )  LIAO Yuhua )  CHENG Xiang )  LU Baojun ) )Institute of Cardiology  Union Hospital  Tongji Medical College  Huazhong University of Science  Technology  Wuhan )
Affiliation:ZHANG Jinying 1,2),LIAO Yuhua 1),CHENG Xiang 1),LU Baojun 3) 1)Institute of Cardiology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022 2)Department of Cardiology,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052 3)Department of Cardiology,the Second Affiliated Hospital,Zhengzhou University,Zhengzhou 450003
Abstract:Aim: To study the effects of simvastatin on the mRNA expression and protein production of IL6 in rats after acute myocardial infarction (AMI). Methods: Rats with AMI were treated with normal saline or simvastatin (gastric gavage) for 4 weeks. The mRNA of IL6 excreted by cardiac myocytes were examined by RTPCR, and IL6 protein levels were detected by Western blot and immunohistochemistry,respectively. The results were compared with that of sham operation group.Results: The mRNA expression and protein production of IL6 in simvastatin or normal saline treatment group were significantly elevated compared with sham operation group (P<0.05). Those in simvastatin group were markedly decreased compared with AMI group treated with normal saline (P<0.05). However, simvastatin did not alter plasma lipids. Conclusion: Simvastatin obviously reduces the gene expression and protein production of IL-6 in rats after AMI, which is not dependent on its lipids-lowering effects.
Keywords:simvastatin  myocardial infarction  interleukin-6  RT-PCR  immunohistochemistry  rat
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