不同类型乙型肝炎患者HBV基因型的分布

目的 探讨乙型肝炎病毒（HBV）各种基因型在不同类型乙型肝炎患者中的分布.方法 用PCR结合限制性片段长度多态性（PCR-RFLP）方法检测56例HBV感染的肝癌、58例肝硬化、60例慢性乙型肝炎患者以及60例HBV无症状携带者血清HBV基因型.结果 无症状携带者中B基因型为46.7%（28/60）,C基因型为33.3%（20/60）,D基因型为20.0%（12/60）;慢性乙型肝炎组B基因型为41.7%（25/60）,C基因型为36.7%（22/60）,D基因型为21.7%（13/60）;肝癌患者中B基因型为33.9%（19/56）、C基因型为60.7%（34/56）、D基因型为5.4%（3/56）;肝硬化患者中B基因型为31.0%（18/58）、C基因型为63.8%（37/58）,D基因型为3.5%（2/58）.结论 在慢性乙型肝炎和无症状携带者中以B基因型为优势感染毒株,而肝硬化和肝癌患者则以C基因型为优势感染毒株.

Distributing of HBV genotypes in patients infected with hepatitis virus B

Objective To investigate the relationship between HBV genotype and the mechanism of liver cirrhosis and liver cancer.Methods Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) were used to detect HBV genotypes in 56 patients with liver cancer,58 patients with liver cirrhosis and 60 patients with chronic hepatitis B.Meanwhile,60 HBV asymptomatic carriers were detected as control.Results Among 60 HBV asymptomatic carriers,the percentages of genotype B,C and D were 46.7%,33.3% and 20.0 % respectively.Among 60 chronic hepatitis B,the percentages of genotype B,C and D were 41.7 %,36.7% and 21.7 % respectively.Among the patients with liver cancer and liver cirrhosis, the percentages of genotype B were 33.9 % and 31.0%, genotype C were 60.7% and 63.8%, genotype D were 5.4% and 3.5%, respectively.Conclusion Genotype B is the main genotype in group of HBV asymptomatic carriers and chronic hepatitis B, and genotype C is the prevailing genotype in the patients with liver cancer and liver cirrhosis. The results suggests that genotype C may correlate to the development of liver diseases.