人参茎叶总皂苷中人参皂苷Re的含量分析

目的：分析人参茎叶总皂苷中人参皂苷R。的含量，为人参总皂苷的质量控制提供依据。方法：色谱柱：Diamonsil C18（4．6mm×25mm，5μm），保护柱：DIKMAEasyGuardC18（10mm×4．6mm），流动相：乙腈-0．5％冰醋酸水溶液，梯度洗脱，流速：1．25mL／min；EISD：漂移管温度40℃，载气压力3．5bar，放大系数为7。结果：标准曲线：C=1．191×10^-7A-0．1876，线性范围：0．038～1．14mg／mL，r=0．9993，检出限：20ng（S／N〉3），加样回收率：96．84％～104．21％。结论：该方法前处理简单，分析准确、快速，可作为人参茎叶总皂苷中人参皂苷Re的含量分析方法。

Determination of ginsenoside R_e in total ginsenoside from stalk and leaf of Panax ginseng

Objective ： To determine quantitatively ginsenoside Re in total ginsenoside from stalk and leaf of Panax ginseng, and to provide experimental evident for total ginsenoside quality control of Panax ginseng. Methods ： The method was developed with the con- dition as follows ： Diamonsil C js （4.6 mm x 25 mm,5 p~m） and DIKMA EasyGuard C ls （10 mm ~ 4.6 mm） was used as chromatograph- ic column and guard column respectively. Gradient elution was employed with the mobile phase of acetonitrile--0.5% acetic acid/water at a flow rate of 1.25 mL/min. ELSD：temperature of the drift tube 40 ℃ ; gas pressure： 3.4 bar; gain： 7. Results：The standard curve ： C -- 1.191 × 10^ - 7A - 0. 187 6 ; the linear range ： 0. 038 - 1.14 mg/mL, r = 0. 999 3 ; the limits of detection ： 20 ng （ S/N 〉 3 ） ; recovery rate of the added sample： 96.84% - 104.21%. Conclusion：With its accuracy, sensitivity, reproducibility as well as the sim- plicity of sample preparation, this method is feasible for quality control of total ginsenoside from stalk and leaf of Panax ginseng.