FOXP3基因多态性与变应性鼻炎的相关性研究

目的 探讨FOXP3基因多态性与变应性鼻炎(allergic rhinitis,AR)易感性的关系.方法 以193例变应性鼻炎患者和191例健康对照人群为研究对象,根据Hapmap网站公布的中国北京地区人群的FOXP3基因标签单核苷酸多态性(single-nucleotide polymorphisms,SNPs)信息及Haploview 4.1软件运行结果,选取rs3761547和rs3761548两个位点,以聚合酶链反应测序法完成基因型的检测,以SPSS13.0软件进行统计学分析.结果 位点rs3761547及rs3761548的杂合突变与AR具有相关性,但只有位点rs3761548经校正P值后仍有统计学意义[比值比(odds ratio,OR)=3.12,95%可信区间(confidence interval,CI)为1.21～8.04,P=0.020].不同过敏原的分层分析显示,尘螨过敏患者中rs3761547与发病具有相关性(OR=2.00,95%CI为1.18～3.39,P=0.013).结论 m3761548的AC杂合突变与AR发病具有相关性,而rs3761547与尘螨过敏的AR相关.

Correlation between FOXP3 gene polymorphisms and allergic rhinitis

Objective To investigate the correlation between polymorphisms in the FOXP3 locus and the susceptibility of allergic rhinitis. Methods Two polymorphisms in FOXP3 locus were genotyped in 193 subjects with allergic rhinitis and 191 healthy controls. The polymorphisms were chosen in terms of the information from Hapmap website as well as Haploview version 4. 1 program implemented results. PCR-resequencing methods was performed for genotyping. SPSS program version 13. 0 was used for statistical analysis. Results Heterozygotes of rs3761547 and rs3761548 appeared significant, while only rs3761548 (P=0.020, OR = 3. 12, 95% CI were 1.21-8.04) remained significant following application of the Bonferroni correction for multiple testing. Subgroup analysis for the presence of different allergen allergy also showed significant association for house dust mites (rs3761547, P=0.013, OR =2.00). Conclusion Heterozygous allele in rs3761548 is associated with allergic rhinitis and rs3761547 is related to house dust mites allergic rhinitis.