| 高效液相色谱法测定左羟丙哌嗪中右旋异构体的含量 |
| |
| 引用本文： | 肖凯,阳海,卜振军.高效液相色谱法测定左羟丙哌嗪中右旋异构体的含量[J].中南药学,2011,9(12):901-904. |
| |
| 作者姓名： | 肖凯 阳海 卜振军 |
| |
| 作者单位： | 1. 湖南省妇幼保健院,长沙,410008
2. 湖南九典制药有限公司,长沙,410011 |
| |
| 摘 要： | 目的 建立高效液相色谱法测定左羟丙哌嗪中右旋异构体的含量.方法 色谱柱为CHIRALCEL-OD-H手性柱,流动相为正己烷-异丙醇-二乙胺(90∶10∶0.1),检测波长为250 nm,流速为1.0 mL·min-1,柱温为30℃,进样量为20 μL.结果 右羟丙哌嗪检测浓度的线性范围为2.0～40.0 μg·mL-1,r=0.999 7,平均回收率为99.1％,RSD为0.72％.结论 本法操作简便、灵敏、准确、专属性强,可用于左羟丙哌嗪质量控制.
|
| 关 键 词： | 左羟丙哌嗪 右旋异构体 含量测定 高效液相色谱法 手性柱 |
Determination of dextroisomer in levodropropizine by HPLC |
| |
| XIAO Kai
,
YANG Hai
,
PU Zhen-jun.Determination of dextroisomer in levodropropizine by HPLC[J].Central South Pharmacy,2011,9(12):901-904. |
| |
| Authors: | XIAO Kai YANG Hai PU Zhen-jun |
| |
| Institution: | XIAO Kai1,YANG Hai2,PU Zhen-jun2(1.Maternal and Child Health Hospital of Hunan,Changsha 410008,2.Hunan Jiudian Pharmaceutical Co.,Ltd.,Changsha 410011) |
| |
| Abstract: | Objective To establish a method for the determination of dextroisomer in levodropropizine by HPLC. Methods The chromatographic separation was performed on CHIRALCEL-ODH, the mobile phase consisted of hex- ane-isopropanol-diethylaming （90 ： 10 ： 0. 1）, the detection wavelength was 237 nm, the flow rate was 1.0 mL · min^-1 , the column temperature was 30 ℃, and the inejection volume was 20μL. Results The linear range of levo- dropropizine was 2.0--40.0μg · mL^-1 （r=0. 999 7）, and the average recovery was 99.1%, RSD= O. 72%. Con- clusion The method is simple, sensitive, accurate and high specific, which is suitable for the quality control of levo- dropropizine. |
| |
| Keywords: | levodropropizine dextroisomercontent determination HPLC chiral column |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
