人参皂苷Rg3对培养人瘢痕疙瘩成纤维细胞增殖活性的影响

目的：研究人参皂苷Rg3(GS-Rg3)对体外培养人瘢痕疙瘩的直接抑制作用及可能机制。方法：通过活细胞计数法与MTT比色法分别检测不同浓度GS-Rg3(25、50、100和200 mg·L^-1)在不同作用时间(24、48和72 h)对成纤维细胞增殖活性的影响。 结果：与对照组相比GS-Rg3对瘢痕疙瘩成纤维细胞有抑制作用，随着时间延长及浓度增加，抑制作用有增加趋势。最大抑制浓度为100 mg·L^-1。 结论：GS-Rg3对体外培养人瘢痕疙瘩成纤维细胞有抑制作用，且随着时间延长及浓度增加，其抑制作用增强。

Effect of Ginsenoside-Rg3 on proliferation activity of human keloid fibroblasts

Objective To study the direct inhibition of Ginsenoside-Rg3(GS-Rg3) on human keloid fibroblasts cultivated in vitro and its possible mechanism.Methods The effects of GS-Rg3 with different concentrations(25,50,100 and 200 mg·L~(-1)) on proliferation activity of fibroblast hyperplasia at different time were detected by viable count and MTT chromometry.Results Compared with control group,GS-Rg3 inhibited the proliferation of keloid fibroblasts.The inhibition increased with the prolongation of time and the increasing of concentration.And the max inhibitory concentration was 100 mg·L~(-1).Conclusion Gs-Rg3 can inhibit the proliferation of human keloid fibroblasts cultivated in vitro.And the inhibition will increase with the prolongation of time and the increasing of concentration.