吴茱萸水提物对乙醇造成的大鼠胃损伤的保护作用

目的：研究吴茱萸水提物对乙醇造成的大鼠急性胃损伤的保护作用及其可能的机制。方法：大鼠经腹腔注射424.8,141.6,47.6 mg·kg^-1 3种不同剂量的吴茱萸水提物后,用ex-vivo chamber法测定50%乙醇造成胃损伤前后的胃电压（PD）变化；另外,经在体胃内插管收集灌流液后,采用硝酸还原酶法测定灌流液中一氧化氮的代谢产物（NO_x）的含量,并通过形态测量法测定胃损伤程度。结果：与对照组比较,不同剂量的吴茱萸水提物对促进PD的恢复和降低损伤指数均有显著作用（P<0.05）,并呈现出剂量依赖性。吴茱萸水提物能剂量依赖性地升高胃损伤前后灌流液中NO_x的水平；尤其当剂量为424.8 mg·kg^-1时,与对照组比较,灌流液NO_x的含量显著增加（P<0.01）。结论：吴茱萸水提物对50%乙醇造成的大鼠急性损伤具有较好的保护作用；其机制与增强胃黏膜屏障功能,促进胃黏膜内源性NO合成有关。

Protective effects of Evodia rutaecarpa water extract on ethanol-induced rat gastric lesions

OBJECTIVE: To investigate the protective effects of Evodia rutaecarpa water extract on ethanol-induced acute gastric lesions in rats. METHOD: In this study, animals were intraperitoneally pretreated with vehicle (normal saline) or E. rutaecarpa at 424.8, 141.6, 47.2 mg x kg(-1). Three hours later, gastric lesions were induced by topical application of 50% ethanol for 10 min. The rat gastric transmucosal potential difference (PD) was recorded continuously by ex-vivo chamber technique. NO(x) (nitrate and nitrite) level in gastric perfusate and the length index of gastric lesions were determined in a gastric intubatton model. RESULT: Compared with control, Evodia rutaecarpa water extract accelerated PD recovery and reduced gastric morphologic lesions in a dose-dependent manner (P < 0.05) , and also dose-dependently increased NO(x) level in gastric perfusate. Especially, at 424.8 mg x kg(-1), E. rutaecarpa promoted synthesizing of nitric oxide significantly (P < 0. 01). CONCLUSION: E. rutaecarpa water extract showed effectively protective actions on ethanol-induced acute gastric lesions in rats, and the gastroprotective mechanisms maybe due to the strengthening action on gastric mucosal lining and the promotion of nitric oxide synthesis in local gastric mucosa.