体外培养的肝癌细胞株与正常肝细胞株蛋白质的差异表达

目的:运用SELDI蛋白质芯片技术分析体外培养的肝癌细胞株(HepG2)与正常肝细胞株(L02)蛋白质表达差异．方法:在体外培养HepG2和L02细胞株,收获细胞,将细胞用细胞裂解液裂解后,采用SELDI蛋白质芯片技术用IMAC3 及WCX2芯片检测HepG2、L02的蛋白质谱．结果:体外培养的肝癌细胞株与正常肝细胞株的蛋白质存在差异表达,IMAC3芯片共捕获61个蛋白,发现差异峰7个,与 L02细胞相比,其中3个差异蛋白在肝癌细胞中高表达,4个差异蛋白在肝癌细胞中低表达．WCX2芯片共捕获91个蛋白, 发现差异峰14个,其中3个差异蛋白在肝癌细胞中高表达,11 个差异蛋白在肝癌细胞中低表达．结论:SELDI蛋白芯片技术检测肝癌细胞株与正常肝细胞株蛋白质的差异表达方法简便,敏感性高,重复性好．

Differential protein expression between in vitro cultured liver cancer cell line HepG2 and normal cell line L02

AIM: To investigate the differentially expressed proteins between liver cancer cell line HepG2 and normal cell line L02 cultured in vitro using surface enhanced laser desorption ionization (SELDI) protein array technology. METHODS: The in vitro cultured liver cancer cell line HepG2 and normal cell line L02 were harvested and then split by cell lysate solution. The protein mass spectra of the HepG2 and L02 cells were detected by IMAC3 and WCX2 based on SELDI protein array technology. RESULTS: A total of 61 proteins were captured by IMAC3 array, and 7 different peaks were found. Compared with those in the L02 cells, 3 differential proteins were highly expressed, while 4 were lowly expressed in the HepG2 cells. A total of 91 proteins were captured by WCX2 array, and 14 differential peaks were found. Among those differentially expressed proteins, 3 were highly expressed, while 11 were lowly expressed in the HepG2 cells. CONCLUSION: SELDI protein array technology is convenient, highly sensitive, and repeatable in the detection of the differently expressed proteins between hepat-ocarcinoma cells and normal hepatocytes.