DDAH1对心肌梗死大鼠心室的重构作用及机制

目的观察DDAH1在心肌梗死模型大鼠中的表达,探索DDAH1对心室的重构作用及机制。方法选取120只DDAH1^KO大鼠,建立心梗模型后随机分为对照组、假手术组和手术组,每组40只。分别于造模前及造模后第7、14、28天观察左室射血分数、左室舒张末期内径、左室收缩末期内径,采用聚合酶链式反应检测eNOS mRNA、PKG mRNA的表达,蛋白印迹法检测eNOS、sGC蛋白的表达。结果假手术组左室射血分数显著下降,左室舒张末期内径和左室收缩末期内径增大。手术组左室射血分数提高,左室舒张末期内径和左室收缩末期内径减小。与对照组同期比较,假手术组第14、28天eNOS、sGC表达差异均有统计学意义(均P<0.05);手术组第14天eNOS、sGC表达均升高(P<0.05),第28天eNOS、sGC表达均较第14天回落。与假手术组同期比较,手术组第28天eNOS、sGC表达差异均有统计学意义(均P<0.05)。结论 DDAH1在心肌梗死中能提高左心室功能,与eNOS、sGC表达有关。

Remodeling Effect and Mechanism of DDAH1 on Ventricles in Rats with Myocardial Infarction

Objective To observe the expression of DDAH1 in rats with myocardial infarction,and to explore the role of DDAH1 in ventricular remodeling and its mechanism.Methods One hundred and twenty DDAH1 KO rats were randomly divided into the control group,sham operation group and operation group,with 40 rats in each group.The left ventricular ejection fraction(LVEF),left ventricular end-diastolic diameter(LVEDD)and left ventricular end-systolic diameter(LVESD)were observed before and after modeling,while the expressions of eNOS mRNA and PKG mRNA were detected by polymerase chain reaction.Western blotting was used to detect the expressions of eNOS and sGC proteins.Results The sham-operated group showed a significant decrease in LVEF and a significant increase in LVEDD and LVESD.The LVEF significantly increased while LVEDD,LVESD significantly decreased in the operation group.Compared with the control group,the expressions of eNOS and sGC of the sham operation group on the 14th and 28th day were significantly different(P<0.05).The expressions of eNOS and sGC were increased in the operation group on the 14th day(P<0.05),but were decreased on the 28th day,and the difference was statistically significant(P<0.05).Compared with the sham operation group,the expression of sGC on the 28th day was significantly different in the operation group(P<0.05).Conclusion DDAH1 can improve left ventricular function in case of myocardial infarction,which is related to the expression of eNOS and sGC.