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| Apoptin基因通过激活caspase-3诱导人黑色素瘤细胞A375凋亡 | |
| 引用本文： | 董萍,咸丰,赵洪礼,李红扬. Apoptin基因通过激活caspase-3诱导人黑色素瘤细胞A375凋亡[J]. 细胞与分子免疫学杂志, 2006, 22(3): 293-295 |
| 作者姓名： | 董萍 咸丰 赵洪礼 李红扬 |
| 作者单位： | 1. 沈阳军区大连疗养院,辽宁,大连,116013 2. 沈阳军区联勤部疾病预防控制中心分子生物学实验室,辽宁,沈阳,110034 3. 皇姑区妇婴医院妇产科,辽宁,沈阳,110031 |
| 摘 要： | 目的研究caspase-3在肿瘤特异性凋亡基因诱导人黑色素瘤细胞A375凋亡中的作用。方法用含有apoptin基因的真核表达载体瞬间转染体外培养的人黑色瘤细胞A375;采用RT-PCR、DNA凝胶电泳、流式细胞术检测A375细胞的凋亡;以比色法检测caspase-3的相对活性。结果Ap-optin基因瞬间转染的A375细胞可出现典型的细胞凋亡所具有的DNA梯状带;流式细胞术发现实验组细胞凋亡率明显高于其他各组(P<0.01);转染后24h实验组caspase-3的活性开始升高,72h达高峰,明显高于其他各组(P<0.01)。结论Apoptin基因可通过激活caspase-3诱导人黑色素瘤细胞A375凋亡。 |
| 关 键 词： | 肿瘤特异性凋亡基因 细胞凋亡 caspase-3[文献标识码A |
| 文章编号： | 1007-8738（2006）03-0293-03 |
| 收稿时间： | 2005-11-04 |
| 修稿时间： | 2005-12-12 |
Apoptin-induced apoptosis of human melanoma cells A375 via activation of caspase-3 |
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| DONG Ping,XIAN Feng,ZHAO Hong-li,LI Hong-yang. Apoptin-induced apoptosis of human melanoma cells A375 via activation of caspase-3[J]. Chinese journal of cellular and molecular immunology, 2006, 22(3): 293-295 | |
| Authors: | DONG Ping XIAN Feng ZHAO Hong-li LI Hong-yang |
| Affiliation: | 1.First Sanatorium of Dalian, Shenyang Military Command, Dalian 116013; 2.Lab. Molecular Biology, Center of Diseases Prevention and Control, Shenyang Military Command, Shenyang 110034; 3.Hospital for Gynecology and Obstetrics of Huanggu District, Shenyang 110031, China |
| Abstract: | AIM: To study whether apoptin gene induces apoptosis in human melanoma cells A375 via the activation caspase-3. METHODS: The human melanoma cells A375 were transiently transfected with recombinant pcDNAA3 plasmid, which contained apoptin gene, and the apoptosis was measured by DNA agarose electrophoresis, RT-PCR and flow cytometry. caspase-3 relative activity was analyzed by colorimetric assay at various time. RESULTS: At 48 h after transfection the apoptin gene could induce apoptosis of human melanoma cells A375 in vitro and the mRNA of apoptin gene could be detected by RT-PCR. Caspase-3 activity began to rise at 24 h after transfection and reached the peak at 72 h. CONCLUSION: Apoptin gene can induce apoptosis in human melanoma cells A375 via active caspase-3 and the activity of caspase-3 is time-dependent. |
| Keywords: | apoptin apoptosis caspase-3 |
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