简易小鼠镇咳实验法

本文报告了一项简易的小鼠镇咳实验方法,系利用恒压条件下喷氢氧化铵气雾,引起小鼠咳嗽反应,并以引起半数小鼠咳嗽的喷雾时间(EDT₅₀)为指标。本法能比较稳定地反映出已知止咳药的作用和作用强度。曾用本法筛选出杜鹃酮、止咳酮,金丝桃甙等得到临床证实的新止咳药。本法简便、快速、耗药量少,节省动物,适用于作大量筛选试验。

A SIMPLE METHOD FOR TESTING ANTITUSSIVE AGENTS IN MICE

A simple and rapid method using mice for testing antitussive agents is reported. Mice were put into a small glass chamber, one by one, and exposed to atomized ammonium hydroxide for various lengths of time. By using a sequential test design the time of exposure that makes 50 per cent of mice cough(EDT₅₀) could be easily obtained. Agents which significantly prolong EDT₅₀ are considered to be potential antitussives.This method has been shown to be suitable to demonstrate the activity and potency of known antitussive drugs such as codeine, toclas, etc. Compounds, including germacrone, 4-phenyl-hutanone-2 and hyperin which were found in our laboratory to exhibit satisfactory antitussive activity by this method, were also shown to be effective in controling cough clinically.