栎精对葡萄糖-6-磷酸酶基因表达及活性的影响

目的：探讨栎精对葡萄糖-6-磷酸酶基因表达及活性的影响。方法：将原代培养的大鼠肝细胞在含有25mmol／L的葡萄糖和不同浓度的栎精培养16h后提取RNA,以半定量RT—PCR方法检测葡萄糖-6-磷酸酶的催化亚基（G6PC）和转运亚基（G6PT）的mRNA水平,并应用葡萄糖双脱氢酶偶联法测定葡萄糖-6-磷酸酶的活性。结果：25mmol／L葡萄糖能够使G6PC和G6PT的mRNA水平升高2倍左右（P〈0．05）,不同浓度的栎精可抑制G6PC和G6PT的转录及酶的活性。结论：栎精能够抑制体外葡萄糖-6-磷酸酶基因的表达及其活性。

Effect of Quercetin on Expression and Activity of Glucose-6-Phosphatase Gene

Objective:To study the effect of quercetin on expression and activity of glucose-6-phosphatase gene.Methods:The primary hepatocyte of rat was cultured in 25 mmol/L glucose and different concentrations of quercetin.Total RNA was extracted after 16 h incubated with 1640 medium containing glucose and quercetin.The levels of mRNA expression of glucose-6-phosphatase catalytic subunit(G6PC)and glucose-6-phosphate translocase(G6PT)were measured by semiquantitative RT-PCR.The enzymatic activity of glucose-6-phosphatase gene was determined by glucose dehydrogenase-coupled reaction.Results:The levels of G6PC and G6PT mRNA increased about two fold after incubated with 25 mmol/L glucose(P < 0.05).The enzymatic activities of G6PC and G6PT mRNA were inhibited by quercetin at different concentrations.Conclusion:Quercetin can inhibit the expression and activity of glucose-6-phosphatase gene induced by glucose in vitro.