芩仙胶囊治疗支气管哮喘的主要药效学研究

目的:观察芩仙胶囊平喘、止咳、化痰和抗炎作用.方法:大鼠50只分5组,生理盐水组(等容量)、芩仙胶囊高(4.59 g·kg-1)、中(2.30 g·kg-1)、低(1.15 g·kg-1)剂量组,氨茶碱(50 mg· kg-1)组,采用卵蛋白引喘法测定哮喘潜伏期；小鼠50只分为5组,生理盐水组(等容量)、芩仙胶囊高(6.63 g·kg-1)、中(3.32 g·kg-1)、低(1.66 g·kg-1)剂量组、复方甘草片组(0.14 g·kg-1)或氯化铵(0.12 g·kg-1),采用浓氨水喷雾测定咳嗽潜伏期和3 min内的咳嗽次数,采用气管段酚红排泌法测定酚红排泌量；小鼠50只分为5组,生理盐水组(等容量)、芩仙胶囊高(6.63 g·kg-1)、中(3.32 g·kg-1)、低(1.66 g·kg-1)剂量组、地塞米松组(2 mg·kg-1),采用二甲苯致小鼠耳廓肿胀法测定肿胀度,采用小鼠皮下肉芽肿增生模型测定肉芽质量,综合研究芩仙胶囊治疗支气管哮喘的主要药效.结果:芩仙胶囊高、中、低剂量组大鼠哮喘潜伏期明显延长(P＜0.05),高、中、低剂量组小鼠咳嗽次数明显减少、咳嗽潜伏期明显延长(P＜0.01,P＜0.05),高、中剂量组小鼠气管段酚红排泌量明显增加(P＜0.05)、耳廓肿胀度明显减轻(P＜0.01,P＜0.05),高、中、低剂量组小鼠琼脂肉芽肿生长明显减少(P＜0.01).结论:芩仙胶囊具有一定的平喘止咳化痰和抗炎作用,对支气管哮喘具有治疗作用.

Main Pharmacodynamics Research of Qinxian Capsules in the Treatment of Bronchial Asthma

Objective: To observe the main effects of the Qinxian capsule in the treatment on asthma, cough, phlegm and anti-inflammatory. Method: Fifty rats were divided into 5 groups, the normal group, Qinxian capsules high (4.59 g·kg^-1), middle(2.30 g·kg^-1), low (1.15 g·kg^-1), Aminophylline (50 mg·kg^-1), by Ovalbumin induced asthma for the determination of the incubation period of asthma;50 mice divided into 5 group, the normal group Qinxian capsules high (6.63 g·kg^-1), middle (3.32 g·kg^-1), low (1.66 g·kg^-1) dose group, Fu fang Gancao tablets group (0.14 g·kg^-1) or ammonium chloride (0.12 g·kg^-1), with ammonia water spray determination of cough incubation period and 3 min cough frequency, determining phenolsulfonphthalein excretion quantity;50 mice were divided into 5 groups, the normal group, Qinxian capsules high (6.63 g·kg^-1), middle (3.32 g·kg^-1), low (1.66 g·kg^-1) dose group, Dexamethasone group (2 mg·kg^-1), using xylene induced ear swelling and determining the swelling degree, using Granuloma hyperplasia model for determination of granulation quality, comprehensive study on the main pharmacodynamics of capsule in treating bronchial asthma. Result: Qinxian capsules high, low dose group of rats with asthma latency was significantly prolonged (P<0.05), high, low dose group of mice cough frequency was significantly reduced and latency was significantly prolonged (P<0.01, P<0.05), high, middle dose group mouse tracheal phenol red excretion increased significantly (P<0.05), auricle swelling degree significantly reduced (P<0.01, P<0.05), high, low dose group mice agar granuloma growth was significantly reduced (P<0.01). Conclusion: Qinxian capsules has reducing cough and resolving phlegm and anti-inflammatory effects, on bronchial asthma with therapeutic effect.