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木蹄复方体外抗肿瘤作用的实验研究
引用本文:何晓义,沈先荣,刘琼,蒋定文,王庆蓉,葛卫红. 木蹄复方体外抗肿瘤作用的实验研究[J]. 中国实验方剂学杂志, 2013, 19(3): 188-191
作者姓名:何晓义  沈先荣  刘琼  蒋定文  王庆蓉  葛卫红
作者单位:1. 浙江中医药大学中药体外代谢实验室,杭州,310053
2. 海军医学研究所防护医学研究室,上海,200433
基金项目:全军医学科技"十二五"重大项目(AS11J003)
摘    要:
目的:筛选木蹄复方抗肿瘤活性提取物,初步分析其化学成分并观察其体外抗肿瘤效应.方法:常规水提和醇提的方法提取复方中水溶和醇溶性组分.MTT法测定木蹄复方水提取物(distilled water extract of compound Fomes fomentarius,WECF)和木蹄复方乙醇提取物(ethanol extract of compound F.fomentarius,EECF)对体外培养肿瘤细胞A549,Hela,QGY生长的抑制效应.系统预试法分析WECF化学组成.流式细胞术检测WECF对A549细胞凋亡的影响.结果:WECF对A549,Hela,QGY 3种细胞的IC50分别为(0.28±0.02),(0.40 ±0.06),(0.28±0.05) g·L-1;EECF对3种细胞的IC50分别为(0.50±0.04),(0.50±0.03),(0.60 ±0.06)g·L-1,WECF对肿瘤细胞的生长具有较强的抑制作用.WECF含有有机酸,氨基酸、多肽和蛋白质,糖、多糖和苷类,皂苷,内酯、香豆素及其苷类,植物甾醇、三萜成分以及挥发油和油脂,多糖含量为(16.1±0.5)%.WECF对A549,Hela,QGY,DU145,MDA-MB-435S,SGC-79016种细胞的IC50分别为0.29,0.45,0.32,0.32,0.83,0.23g·L-1; WECF 1 g·L-1作用于A549 24 h后,细胞凋亡率为22.0%.结论:WECF具有较强的体外抗肿瘤效果,对多种肿瘤细胞的生长具有非常明显的抑制作用,并可诱导A549细胞凋亡.

关 键 词:木蹄层孔菌  赤芝  松针层孔菌  复方  抗肿瘤
收稿时间:2012-08-03

Antitumor Effect in vitro of Compound Fomes fomentarius
HE Xiao-yi,SHEN Xian-rong,LIU Qiong,JIANG Ding-wen,WANG Qing-rong and GE Wei-hong. Antitumor Effect in vitro of Compound Fomes fomentarius[J]. China Journal of Experimental Traditional Medical Formulae, 2013, 19(3): 188-191
Authors:HE Xiao-yi  SHEN Xian-rong  LIU Qiong  JIANG Ding-wen  WANG Qing-rong  GE Wei-hong
Affiliation:Metabolism Laboratory of Traditional Chinese Medicine, Zhejiang Chinese Medicine University, Hangzhou 310053, China;Department of Radiation Protection Medicine, Navy Medical Research Institute, Shanghai 200433, China;Department of Radiation Protection Medicine, Navy Medical Research Institute, Shanghai 200433, China;Department of Radiation Protection Medicine, Navy Medical Research Institute, Shanghai 200433, China;Department of Radiation Protection Medicine, Navy Medical Research Institute, Shanghai 200433, China;Metabolism Laboratory of Traditional Chinese Medicine, Zhejiang Chinese Medicine University, Hangzhou 310053, China
Abstract:
Objective:To screen the antitumor extract of compound Fomes fomentarius, detect its chemical composition preliminarily and evaluate its antitumor activity in vitro. Method: Distilled water or ethanol was used for extraction of compound F. fomentarius. The MTT assay was used to measure the inhibition effect in vitro of distilled water extract of compound F. fomentarius (WECF) and ethanol extract of compound F. fomentarius (EECF). The system pre-test method was used to detect the chemical composition preliminarily. Apoptosis of A549 was tested by flow cytometry. Result: The 50% inhibition concentration (IC50) of WECF to A549, Hela and QGY were (0.28±0.02), (0.40±0.06), (0.28±0.05) g·L-1 respectively, and to EECF, the IC50 were (0.50±0.04), (0.50±0.03), (0.60±0.06) g·L-1 respectively. There were organic acid, amino acid, peptides and proteins, sugar, polysaccharide and nucleoside, saponins, lactone, coumarone and nucleoside, plant sterol and terpene composition, volatile oil and grease in WECF. The polysaccharide content of WECF was (16.1±0.5)%. The IC50 of WECF to A549, Hela, QGY, DU145, MDA-MB-435S and SGC-7901 were 0.29, 0.45, 0.32, 0.32, 0.83, 0.23 g·L-1. 22.0% of totle cells apoptosis after A549 treating with WECF at 1 g·L-1 for 24 h. Conclusion: WECF showed higher antitumor activity in vitro compared with EECF and has significant inhibitory effect to a variety of tumor cells, further investigation revealed that WECF exerted an antitumor activity via apoptosis-induced cell death and maybe developed as a new antitumor medicine.
Keywords:Fomes fomentarius  Ganoderma lucidum  Phellinus igniarius  compound  antitumor
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