大鼠肺再灌注损伤早期外周血中性粒细胞膜CD18的变化

目的：观察大鼠肺再灌注损伤早期外周血中性粒细胞(PMNS)膜CD18的变化。方法：SD大鼠20只，随机分为正常对照组、缺血组、再灌注30min组及再灌注60min组，测定各组左肺湿/干质量(mW/mD)比及肺动脉压，取颈动脉血观察PMNS CD18水平的变化，计数PMNS。结果：再灌注30min组与60 min组左肺mW/mD比及肺动脉压均较对照组明显增高(P<0.01)；再灌注30min组PMNS膜CD18水平较对照组明显升高(P<0.01)；再灌注60min组较再灌注30min组又进一步增高(P<0.01)；再灌注60min组外周血PMNS计数较对照组减少(P<0.05)。结论：大鼠肺再灌注损伤早期外周血PMNS膜CD18水平增高，PMNS计数减少。

The change of PMNS CD18 at the early stage of rat lung reperfusion injury

Objective:To observe the change of peripheral PMNS CD18 at the early stage of rat lung reperfusion injury.Methods:Twenty SD rats were divided randomly into 4 groups:control group,ischemia group,reperfusion 30 min group and reperfusion 60 min group.The left lung wet/dry mass(mW/mD) ratio,the pulmonary artery pressure and the level of circulating PMNS CD18 of animals in each group were measured. Results:The mW/mD ratio and the pulmonary artery pressure of both the reperfusion 30min and 60min group were higher than those of the control group(P<0.01),respectively.The level of PMNS CD18 of the reperfusion 60min group increased significantly compared with that of the reperfusion 30min group(P<0.01).The PMNS count of the circulating blood was lower in the reperfusion 60 min group than in the control group (P<0.05).Conclusion:The level of PMNS CD18 increases and the count of PMNS decreases at the early stage of lung reperfusion injury.